دورية أكاديمية
Purification of human respiratory syncytial virus: superiority of sucrose gradient over percoll, renografin, and metrizamide gradients.
| العنوان: | Purification of human respiratory syncytial virus: superiority of sucrose gradient over percoll, renografin, and metrizamide gradients. |
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| المؤلفون: | Mbiguino A; Laboratory of Immunovirology, Department of Microbiology & Immunology, University of Montreal, Quebec, Canada., Menezes J |
| المصدر: | Journal of virological methods [J Virol Methods] 1991 Feb-Mar; Vol. 31 (2-3), pp. 161-70. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print Cited Medium: Print ISSN: 0166-0934 (Print) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | Diatrizoate Meglumine* , Metrizamide* , Povidone* , Silicon Dioxide*, Centrifugation, Density Gradient/*methods , Respiratory Syncytial Viruses/*isolation & purification, Carcinoma ; Cell Line ; Culture Media ; Humans ; Laryngeal Neoplasms ; Osmotic Pressure ; Respiratory Syncytial Viruses/growth & development ; Virus Activation ; Viscosity |
| مستخلص: | A method was devised for producing and purifying human respiratory syncytial virus (HRSV) preparations with high titers. Previous attempts to obtain substantial amounts of purified human respiratory syncytial virus have been unsuccessful due to the extreme lability of this virus, its close association with the host cell membrane, and its tendency to aggregate during concentration procedures. We describe a comparative study of various purification media as well as a novel approach for obtaining high titers of HRSV. Virus was produced in HEp-2 cells grown on gelatin beads and concentrated either by precipitation with polyethylene glycol or by centrifugation over a sucrose cushion, the latter yielding 100% recovery. These procedures employed EDTA to disaggregate the virus and MgSO4 as a virus stabilizing agent. Attempts to purify HRSV over percoll, renografin and metrizamide gradients lead to loss of infectivity. Sucrose was found to represent the best purification medium with a yield of up to 60%. |
| المشرفين على المادة: | 0 (Culture Media) 3X9MR4N98U (Diatrizoate Meglumine) 65455-52-9 (Percoll) 7631-86-9 (Silicon Dioxide) FZ989GH94E (Povidone) RHH3W8F1CO (Metrizamide) |
| تواريخ الأحداث: | Date Created: 19910201 Date Completed: 19910912 Latest Revision: 20220410 |
| رمز التحديث: | 20221208 |
| DOI: | 10.1016/0166-0934(91)90154-r |
| PMID: | 1650782 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0166-0934 |
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| DOI: | 10.1016/0166-0934(91)90154-r |