دورية أكاديمية
Proteomic identification of the wt-p53-regulated tumor cell secretome.
| العنوان: | Proteomic identification of the wt-p53-regulated tumor cell secretome. |
|---|---|
| المؤلفون: | Khwaja FW; Laboratory of Molecular Neuro-Oncology, Department of Neurosurgery, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA., Svoboda P, Reed M, Pohl J, Pyrzynska B, Van Meir EG |
| المصدر: | Oncogene [Oncogene] 2006 Dec 07; Vol. 25 (58), pp. 7650-61. Date of Electronic Publication: 2006 Oct 09. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8711562 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0950-9232 (Print) Linking ISSN: 09509232 NLM ISO Abbreviation: Oncogene Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002->: Basingstoke : Nature Publishing Group Original Publication: Basingstoke, Hampshire, UK : Scientific & Medical Division, MacMillan Press, c1987- |
| مواضيع طبية MeSH: | Cell Transformation, Neoplastic* , Gene Expression Regulation, Neoplastic* , Proteome*, Glioblastoma/*metabolism , Neoplasm Proteins/*metabolism , Tumor Suppressor Protein p53/*physiology, Cell Line, Tumor ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Isotope Labeling ; Protein Processing, Post-Translational ; Protein Transport |
| مستخلص: | Tumor-stroma interactions play a major role in tumor development, maintenance and progression. Yet little is known on how the genetic alterations that underlie cell transformation elicit cell extrinsic changes modulating heterotypic cell interactions. We hypothesized that these events involve a modification in the complement of secreted proteins by the cell, acting as mediators of intercellular communication. To test this hypothesis, we examined the role of wt-p53, a major tumor suppressor, on the tumor microenvironment through its regulation of secreted factors. Using a combination of 2-DE and cICAT proteomic techniques, we found a total of 111 secreted proteins, 39 of which showed enhanced and 21 inhibited secretion in response to wt-p53 expression. The majority of these were not direct targets of p53 transcription factor activity, suggesting a novel role for wt-p53 in the control of intracellular protein trafficking and/or secreted protein stability. Evidence for p53-controlled post-translational modifications on nine secreted proteins was also found. These findings will enhance our understanding of wt-p53 modulated interactions of the tumor with its environment. |
| معلومات مُعتمدة: | CA 86335 United States CA NCI NIH HHS; RR 02878 United States RR NCRR NIH HHS; RR 12878 United States RR NCRR NIH HHS; RR 13948 United States RR NCRR NIH HHS |
| المشرفين على المادة: | 0 (Neoplasm Proteins) 0 (Proteome) 0 (Tumor Suppressor Protein p53) |
| تواريخ الأحداث: | Date Created: 20061018 Date Completed: 20070116 Latest Revision: 20220310 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1038/sj.onc.1209969 |
| PMID: | 17043663 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!