دورية أكاديمية
Lunatic fringe controls T cell differentiation through modulating notch signaling.
| العنوان: | Lunatic fringe controls T cell differentiation through modulating notch signaling. |
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| المؤلفون: | Tsukumo S; Department of Immunology and Parasitology, Institute of Health Biosciences, University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan., Hirose K, Maekawa Y, Kishihara K, Yasutomo K |
| المصدر: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2006 Dec 15; Vol. 177 (12), pp. 8365-71. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Print ISSN: 0022-1767 (Print) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bethesda, MD : American Association of Immunologists Original Publication: Baltimore : Williams & Wilkins, c1950- |
| مواضيع طبية MeSH: | Cell Differentiation*, Glycosyltransferases/*physiology , Receptors, Notch/*metabolism , Signal Transduction/*physiology , T-Lymphocytes/*cytology, Animals ; CD4 Antigens ; CD8 Antigens ; Gene Expression Regulation ; Humans ; Jurkat Cells ; Mice ; Mice, Inbred C57BL ; Thymus Gland/cytology |
| مستخلص: | T cells differentiate from bone marrow-derived stem cells by expressing developmental stage-specific genes. We here searched arrays of genes that are highly expressed in mature CD4-CD8+ (CD8 single-positive (SP)) T cells but little in CD4+CD8+ (double-positive (DP)) cells by cDNA subtraction. Lunatic fringe (Lfng), a modulator of Notch signaling, was identified to be little expressed in DP cells and highly expressed in CD8SP T cell as well as in CD4-CD8- (double-negative (DN)) and mature CD4+CD8- (CD4SP) T cells. Thus, we examined whether such change of expression of Lfng plays a role in T cell development. We found that overexpression of Lfng in Jurkat T cells strengthened Notch signaling by reporter gene assay, indicating that Lfng is a positive regulator for Notch signaling in T cells. The enforced expression of Lfng in thymocytes enhanced the development of immature CD8SP cells but decreased mature CD4SP and CD8SP cells. In contrast, the down-regulation of Lfng in thymocytes suppressed DP cells development due to the defective transition from CD44+CD25- stage to subsequent stage in DN cells. The overexpression of Lfng in fetal liver-derived hemopoietic stem cells enhanced T cell development, whereas its down-regulation suppressed it. These results suggested that the physiological high expression of Lfng in DN cells contributes to enhance T cell differentiation through strengthening Notch signaling. Shutting down the expression of Lfng in DP cells may have a physiological role in promoting DP cells differentiation toward mature SP cells. |
| المشرفين على المادة: | 0 (CD4 Antigens) 0 (CD8 Antigens) 0 (Receptors, Notch) EC 2.4.- (Glycosyltransferases) EC 2.4.1.- (Lfng protein, mouse) |
| تواريخ الأحداث: | Date Created: 20061205 Date Completed: 20070116 Latest Revision: 20190516 |
| رمز التحديث: | 20221213 |
| DOI: | 10.4049/jimmunol.177.12.8365 |
| PMID: | 17142733 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-1767 |
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| DOI: | 10.4049/jimmunol.177.12.8365 |