دورية أكاديمية
Developmental regulation of DUOX1 expression and function in human fetal lung epithelial cells.
| العنوان: | Developmental regulation of DUOX1 expression and function in human fetal lung epithelial cells. |
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| المؤلفون: | Fischer H; Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609-1673, USA. hfischer@chori.org, Gonzales LK, Kolla V, Schwarzer C, Miot F, Illek B, Ballard PL |
| المصدر: | American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2007 Jun; Vol. 292 (6), pp. L1506-14. Date of Electronic Publication: 2007 Mar 02. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901229 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1040-0605 (Print) Linking ISSN: 10400605 NLM ISO Abbreviation: Am J Physiol Lung Cell Mol Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1989- |
| مواضيع طبية MeSH: | Flavoproteins/*genetics , NADPH Oxidases/*genetics , Respiratory Mucosa/*embryology , Respiratory Mucosa/*physiology, Acids/metabolism ; Cell Differentiation/drug effects ; Cell Differentiation/physiology ; Cells, Cultured ; Dexamethasone/pharmacology ; Dual Oxidases ; Fetus/cytology ; Flavoproteins/metabolism ; Gene Expression Regulation, Developmental ; Glucocorticoids/pharmacology ; Humans ; Hydrogen Peroxide/metabolism ; NADPH Oxidases/metabolism ; Phenotype ; Pulmonary Alveoli/cytology ; Pulmonary Alveoli/embryology ; Pulmonary Alveoli/physiology ; RNA, Messenger/metabolism ; Respiratory Mucosa/cytology ; Up-Regulation/drug effects ; Up-Regulation/physiology |
| مستخلص: | The purpose of this study was to determine the expression and cellular functions of the epithelial NADPH oxidase DUOX1 during alveolar type II cell development. When human fetal lung cells (gestational age 11-22 wk) were cultured to confluency on permeable filters, exposure of cells to a hormone mixture (dexamethasone, 8-Br-cAMP, and IBMX, together referred to as DCI) resulted in differentiation of cells into a mature type II phenotype as assessed by expression of lamellar bodies, surfactant proteins, and transepithelial electrical parameters. After 6 days in culture in presence of DCI, transepithelial resistance (2,616 +/- 529 Omega.cm(2)) and potential (-8.5 +/- 0.6 mV) indicated epithelial polarization. At the same time, treatment with DCI significantly increased the mRNA expression of DUOX1 ( approximately 21-fold), its maturation factor DUOXA1 ( approximately 12-fold), as well as DUOX protein ( approximately 12-fold), which was localized near the apical cell pole in confluent cultures. For comparison, in fetal lung specimens, DUOX protein was not detectable at up to 27 wk of gestational age but was strongly upregulated after 32 wk. Function of DUOX1 was assessed by measuring H(2)O(2) and acid production. Rates of H(2)O(2) production were increased by DCI treatment and blocked by small interfering RNA directed against DUOX1 or by diphenylene iodonium. DCI-treated cultures also showed increased intracellular acid production and acid release into the mucosal medium, and acid production was largely blocked by knockdown of DUOX1 mRNA. These data establish the regulated expression of DUOX1 during alveolar maturation, and indicate DUOX1 in alveolar H(2)O(2) and acid secretion by differentiated type II cells. |
| معلومات مُعتمدة: | HL-071829 United States HL NHLBI NIH HHS; HL-19737 United States HL NHLBI NIH HHS; HL-56401 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Acids) 0 (Flavoproteins) 0 (Glucocorticoids) 0 (RNA, Messenger) 7S5I7G3JQL (Dexamethasone) BBX060AN9V (Hydrogen Peroxide) EC 1.11.1.- (Dual Oxidases) EC 1.6.3.- (NADPH Oxidases) EC 1.6.3.1 (DUOX1 protein, human) |
| تواريخ الأحداث: | Date Created: 20070306 Date Completed: 20070727 Latest Revision: 20200930 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1152/ajplung.00029.2007 |
| PMID: | 17337509 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1040-0605 |
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| DOI: | 10.1152/ajplung.00029.2007 |