دورية أكاديمية
أعرض في EDS

Maturational arrest of thymocyte development is caused by a deletion in the receptor-like protein tyrosine phosphatase kappa gene in LEC rats.

التفاصيل البيبلوغرافية
العنوان: Maturational arrest of thymocyte development is caused by a deletion in the receptor-like protein tyrosine phosphatase kappa gene in LEC rats.
المؤلفون: Kose H; Division of Animal Research Resources, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan., Sakai T, Tsukumo S, Wei K, Yamada T, Yasutomo K, Matsumoto K
المصدر: Genomics [Genomics] 2007 Jun; Vol. 89 (6), pp. 673-7. Date of Electronic Publication: 2007 Apr 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 8800135 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0888-7543 (Print) Linking ISSN: 08887543 NLM ISO Abbreviation: Genomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Diego : Academic Press, [c1987-
مواضيع طبية MeSH: Protein Tyrosine Phosphatases/*genetics , T-Lymphocytes/*metabolism, Animals ; Animals, Congenic ; Base Sequence ; Cell Differentiation ; Chromosome Mapping ; DNA, Complementary/genetics ; Female ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Organ Culture Techniques ; Pregnancy ; Protein Tyrosine Phosphatases/antagonists & inhibitors ; Protein Tyrosine Phosphatases/metabolism ; RNA Interference ; Rats ; Rats, Inbred LEC ; Sequence Deletion ; T-Lymphocytes/cytology ; T-Lymphocytes/immunology ; Thymus Gland/cytology ; Thymus Gland/immunology ; Thymus Gland/metabolism
مستخلص: The Long-Evans Cinnamon (LEC) rat has a spontaneous mutation, T helper immunodeficiency (thid), which causes a markedly reduced CD4(+) thymocyte population. Here we positionally clone the locus and identify a deletion in the gene encoding a receptor-like protein tyrosine phosphatase kappa (Ptprk) that led to complete loss of the transcript. The rat Ptprk gene exhibits 98% identity with the human and mouse counterparts and is expressed most abundantly in the CD4(-)CD8(-) double-negative stage. The downregulation of Ptprk in mouse immature thymocytes by RNA interference mimicked the thid phenotype. These results indicate that thid maps to the Ptprk locus and that functional Ptprk is crucial for lineage commitment or progression of CD4(+) T cells. We also found that Ptprk appears to function in parallel with or downstream of Th-POK/cKrox (also known as ZBTB7B), a master regulator of T cell lineage decision.
سلسلة جزيئية: GENBANK AB288087; AB288088
المشرفين على المادة: 0 (DNA, Complementary)
EC 3.1.3.48 (Protein Tyrosine Phosphatases)
تواريخ الأحداث: Date Created: 20070417 Date Completed: 20070711 Latest Revision: 20071115
رمز التحديث: 20240628
DOI: 10.1016/j.ygeno.2007.03.001
PMID: 17434290
قاعدة البيانات: MEDLINE
الوصف
تدمد:0888-7543
DOI:10.1016/j.ygeno.2007.03.001