دورية أكاديمية
mBAND analysis of chromosomal aberrations in human epithelial cells exposed to low- and high-LET radiation.
| العنوان: | mBAND analysis of chromosomal aberrations in human epithelial cells exposed to low- and high-LET radiation. |
|---|---|
| المؤلفون: | Hada M; Universities Space Research Association, Houston, Texas 77085, USA. megumi.hada1@jsc.nasa.gov, Cucinotta FA, Gonda SR, Wu H |
| المصدر: | Radiation research [Radiat Res] 2007 Jul; Vol. 168 (1), pp. 98-105. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Radiation Research Society Country of Publication: United States NLM ID: 0401245 Publication Model: Print Cited Medium: Print ISSN: 0033-7587 (Print) Linking ISSN: 00337587 NLM ISO Abbreviation: Radiat Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bozeman, MT : Radiation Research Society Original Publication: Charlottesville, VA : Kluge Carden Jennnings Pub. Co. |
| مواضيع طبية MeSH: | Linear Energy Transfer*, Chromosome Aberrations/*radiation effects , Epithelial Cells/*metabolism , Epithelial Cells/*radiation effects, Cell Line ; Color ; Humans ; In Situ Hybridization |
| مستخلص: | Energetic heavy ions pose a potential health risk to astronauts who have participated in extended space missions. High-LET radiation is much more effective than low-LET radiation in the induction of biological effects, including cell inactivation, genetic mutations, cataracts and cancer. Most of these biological end points are closely correlated with chromosomal damage, which can be used as a biomarker for radiation damage. Multicolor banding in situ hybridization (mBAND) has proven to be highly useful for the study of intrachromosomal aberrations, which have been suggested as a biomarker of exposure to high-LET radiation. To investigate biological signatures of radiation quality and the complexity of intrachromosomal aberrations, we exposed human epithelial cells in vitro to (137)Cs gamma rays or iron ions (600 MeV/nucleon) and collected chromosomes using a premature chromosome condensation technique. Aberrations in chromosome 3 were analyzed using mBAND probes. The results of our study confirmed the observation of a higher incidence of inversions for high-LET radiation. However, detailed analysis of the inversion type revealed that both iron ions and gamma rays induced a low incidence of simple inversions. Half of the inversions observed in the low-LET-irradiated samples were accompanied by other types of intrachromosome aberrations, but few inversions were accompanied by interchromosome aberrations. In contrast, iron ions induced a significant fraction of inversions that involved complex rearrangements of both inter- and intrachromosome exchanges. |
| تواريخ الأحداث: | Date Created: 20070829 Date Completed: 20070914 Latest Revision: 20070828 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1667/RR0759.1 |
| PMID: | 17722995 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0033-7587 |
|---|---|
| DOI: | 10.1667/RR0759.1 |