دورية أكاديمية
Laser capture microdissection technology.
| العنوان: | Laser capture microdissection technology. |
|---|---|
| المؤلفون: | Espina V; Center for Applied Proteomics & Molecular Medicine, George Mason University, Manassas, VA 20110, USA. vespina@gmu.edu, Heiby M, Pierobon M, Liotta LA |
| المصدر: | Expert review of molecular diagnostics [Expert Rev Mol Diagn] 2007 Sep; Vol. 7 (5), pp. 647-57. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 101120777 Publication Model: Print Cited Medium: Internet ISSN: 1744-8352 (Electronic) Linking ISSN: 14737159 NLM ISO Abbreviation: Expert Rev Mol Diagn Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Abingdon, Oxford : Taylor & Francis Original Publication: London : Future Drugs Ltd, 2001- |
| مواضيع طبية MeSH: | Lasers*, Microdissection/*instrumentation , Neoplasms/*genetics, Biopsy ; Gene Expression Profiling ; Genome ; Humans ; Microdissection/methods ; Neoplasms/pathology ; Neoplasms/therapy ; Proteins/genetics ; Proteins/isolation & purification ; Proteome ; RNA/genetics ; RNA/isolation & purification ; RNA, Messenger/genetics ; RNA, Messenger/isolation & purification |
| مستخلص: | Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions through molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue microecology. Laser capture microdissection is a method to procure subpopulations of tissue cells under direct microscopic visualization. Laser capture microdissection technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity analysis, RNA transcript profiling, cDNA library generation, mass spectrometry proteomics discovery and signal pathway profiling. |
| Number of References: | 104 |
| معلومات مُعتمدة: | AG20948 United States AG NIA NIH HHS |
| المشرفين على المادة: | 0 (Proteins) 0 (Proteome) 0 (RNA, Messenger) 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20070926 Date Completed: 20071011 Latest Revision: 20071203 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1586/14737159.7.5.647 |
| PMID: | 17892370 |
| قاعدة البيانات: | MEDLINE |
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