دورية أكاديمية
Detection of Enterobacteriaceae producing CTX-M extended spectrum beta-lactamases from a tertiary care hospital in south India.
| العنوان: | Detection of Enterobacteriaceae producing CTX-M extended spectrum beta-lactamases from a tertiary care hospital in south India. |
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| المؤلفون: | Baby Padmini S; School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore-632 014, Tamil Nadu, India. padmiraja@yahoo.com, Appala Raju B, Mani KR |
| المصدر: | Indian journal of medical microbiology [Indian J Med Microbiol] 2008 Apr-Jun; Vol. 26 (2), pp. 163-6. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8700903 Publication Model: Print Cited Medium: Print ISSN: 0255-0857 (Print) Linking ISSN: 02550857 NLM ISO Abbreviation: Indian J Med Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New Delhi : Indian Association of Medical Microbiologists |
| مواضيع طبية MeSH: | Escherichia coli/*drug effects , Escherichia coli/*enzymology , Escherichia coli Infections/*microbiology , Klebsiella Infections/*microbiology , Klebsiella pneumoniae/*drug effects , Klebsiella pneumoniae/*enzymology , beta-Lactamases/*biosynthesis, Anti-Bacterial Agents/pharmacology ; Cephalosporins/pharmacology ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Escherichia coli/isolation & purification ; Hospitals ; Humans ; India ; Klebsiella pneumoniae/isolation & purification ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; beta-Lactam Resistance ; beta-Lactamases/genetics ; beta-Lactamases/metabolism |
| مستخلص: | A total of 23 clinical isolates (15 Escherichia coli and 8 Klebsiella pneumoniae), resistant to cefotaxime and ceftazidime recovered during 2002 and 2003, were investigated for production of CTX-M extended spectrum beta-lactamase (ESBL) by phenotypic and molecular methods. The presence of ESBL was tested by NCCLS phenotypic confirmatory test using cephalosporin/clavulanate combination discs and E-test ESBL strips. Determination of MIC of cefotaxime and ceftazidime was done with and without the presence of clavulanic acid by agar dilution technique. Polymerase chain reaction revealed the presence of CTX-M type ESBLs in 19 isolates. Further sequencing resulted in identification of CTX-M-15 ESBLs. This is the first report identifying CTX-M type ESBL from clinical isolates of E. coli and K. pneumoniae from a tertiary care hospital in south India. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Cephalosporins) 0 (DNA, Bacterial) EC 3.5.2.6 (beta-Lactamases) |
| تواريخ الأحداث: | Date Created: 20080501 Date Completed: 20080807 Latest Revision: 20220408 |
| رمز التحديث: | 20231215 |
| DOI: | 10.4103/0255-0857.40534 |
| PMID: | 18445956 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0255-0857 |
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| DOI: | 10.4103/0255-0857.40534 |