دورية أكاديمية
Schistosoma mansoni: lack of correlation between praziquantel-induced intra-worm calcium influx and parasite death.
| العنوان: | Schistosoma mansoni: lack of correlation between praziquantel-induced intra-worm calcium influx and parasite death. |
|---|---|
| المؤلفون: | Pica-Mattoccia L; Institute of Cell Biology, National Research Council, 32 Via Ramarini, 00015 Monterotondo, Rome, Italy., Orsini T, Basso A, Festucci A, Liberti P, Guidi A, Marcatto-Maggi AL, Nobre-Santana S, Troiani AR, Cioli D, Valle C |
| المصدر: | Experimental parasitology [Exp Parasitol] 2008 Jul; Vol. 119 (3), pp. 332-5. Date of Electronic Publication: 2008 Mar 31. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Anthelmintics/*pharmacology , Calcium/*metabolism , Praziquantel/*pharmacology , Schistosoma mansoni/*drug effects, Animals ; Benzodiazepinones/pharmacology ; Calcium Channel Blockers/pharmacology ; Calcium Radioisotopes ; Cytochalasin D/pharmacology ; Kinetics ; Male ; Mice ; Nicardipine/pharmacology ; Nifedipine/pharmacology ; Nucleic Acid Synthesis Inhibitors/pharmacology ; Schistosoma mansoni/metabolism ; Schistosomicides/pharmacology |
| مستخلص: | The schistosomicidal activity of praziquantel (PZQ) is accompanied by a large influx of calcium into the worms, suggesting that this phenomenon could be the source of the observed muscular contraction, surface disruption and eventual death of the parasite. We have incubated live adult schistosomes in a medium containing radioactive calcium and we were able to confirm that PZQ does indeed stimulate calcium entry into the parasite. An even higher calcium uptake, however, occurred in schistosomes exposed to PZQ after pre-incubation with cytochalasin D, a condition that suppresses PZQ schistosomicidal effects and allows the complete survival of the parasites. The calcium blockers nicardipine and nifedipine also failed to prevent the calcium influx induced by PZQ. Similarly, a large calcium influx occurred in 28-day-old worms exposed to PZQ, in spite of the fact that these immature worms are largely insensitive to the schistosomicidal effects of the drug. Schistosomes incubated overnight with radioactive calcium and PZQ and then returned to normal medium, retained a calcium content higher than worms pre-incubated with cytochalasin D, but the difference could be a consequence--rather than a cause--of schistosomicidal effects. These results suggest that calcium accumulation by itself, at least as measured in whole parasites maintained in vitro, may not represent an exhaustive explanation for the schistosomicidal effects of PZQ. |
| المشرفين على المادة: | 0 (Anthelmintics) 0 (Benzodiazepinones) 0 (Calcium Channel Blockers) 0 (Calcium Radioisotopes) 0 (Nucleic Acid Synthesis Inhibitors) 0 (Schistosomicides) 22144-77-0 (Cytochalasin D) 6490C9U457 (Praziquantel) CZ5312222S (Nicardipine) I9ZF7L6G2L (Nifedipine) RN43209SMA (meclonazepam) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20080506 Date Completed: 20080625 Latest Revision: 20220311 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.exppara.2008.03.012 |
| PMID: | 18456260 |
| قاعدة البيانات: | MEDLINE |
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