دورية أكاديمية
Molecular characterization of two Rocio flavivirus strains isolated during the encephalitis epidemic in São Paulo State, Brazil and the development of a one-step RT-PCR assay for diagnosis.
| العنوان: | Molecular characterization of two Rocio flavivirus strains isolated during the encephalitis epidemic in São Paulo State, Brazil and the development of a one-step RT-PCR assay for diagnosis. |
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| المؤلفون: | Coimbra TL; Seção de Vírus Transmitidos por Artrópodos, Instituto Adolfo Lutz, São Paulo, SP, Brasil. licoimbra@ial.sp.gov.br, Santos RN, Petrella S, Nagasse-Sugahara TK, Castrignano SB, Santos CL |
| المصدر: | Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2008 Mar-Apr; Vol. 50 (2), pp. 89-94. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo Country of Publication: Brazil NLM ID: 7507484 Publication Model: Print Cited Medium: Print ISSN: 0036-4665 (Print) Linking ISSN: 00364665 NLM ISO Abbreviation: Rev Inst Med Trop Sao Paulo Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo |
| مواضيع طبية MeSH: | Disease Outbreaks* , Reverse Transcriptase Polymerase Chain Reaction*, Encephalitis, Viral/*virology , Flavivirus/*genetics , Flavivirus Infections/*virology , Viral Nonstructural Proteins/*genetics, Base Sequence ; Brazil/epidemiology ; DNA Primers/analysis ; Encephalitis, Viral/epidemiology ; Flavivirus/classification ; Flavivirus Infections/epidemiology ; Humans ; Molecular Sequence Data ; Phylogeny ; RNA, Viral/analysis ; Viral Proteins/genetics |
| مستخلص: | Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in São Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007). |
| المشرفين على المادة: | 0 (DNA Primers) 0 (NS5 protein, flavivirus) 0 (RNA, Viral) 0 (Viral Nonstructural Proteins) 0 (Viral Proteins) |
| تواريخ الأحداث: | Date Created: 20080520 Date Completed: 20080829 Latest Revision: 20190608 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1590/s0036-46652008000200005 |
| PMID: | 18488087 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0036-4665 |
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| DOI: | 10.1590/s0036-46652008000200005 |