دورية أكاديمية
أعرض في EDS

Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap.

التفاصيل البيبلوغرافية
العنوان: Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap.
المؤلفون: Bazou D; Cardiff School of Biosciences, Cardiff University, Museum Place, Cardiff CF10 3TL, Wales, UK. Bazoud@cf.ac.uk, Coakley WT, Hayes AJ, Jackson SK
المصدر: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2008 Aug; Vol. 22 (5), pp. 1321-31. Date of Electronic Publication: 2008 Apr 08.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 8712158 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0887-2333 (Print) Linking ISSN: 08872333 NLM ISO Abbreviation: Toxicol In Vitro Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon Press, c1987-
مواضيع طبية MeSH: Alginates* , Ultrasonics*, Carcinoma, Hepatocellular/*pathology , Hepatocytes/*pathology, Actins/metabolism ; Albumins/metabolism ; Biocompatible Materials ; Calcium Chloride ; Cell Aggregation/physiology ; Cell Death ; Cell Proliferation ; Cell Survival/physiology ; Hepatocytes/metabolism ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate
مستخلص: We report proof of principle here of a gel encapsulation technique that departs from the minimum surface area to volume restriction of spherical microcapsules and allows gelation of preformed high-density (>or=2x10(4) cells/aggregate) 3-D HepG2 cell aggregates. The process involves forming a discoid 3-D cell aggregate in an ultrasound standing wave trap (USWT), which is subsequently recovered and encapsulated in alginate/CaCl2 hydrogel. The size of the ultrasound-formed aggregates was dependent upon the initial cell concentration, and was in the range of 0.4-2.6 mm in diameter (for cell concentrations ranging between 10(4) and 5x10(6)/ml). At low cell concentrations (or=10(6)/ml, 3-D aggregates were generated. Cells in non- and encapsulated 3-D HepG2 aggregates remained 70-80% viable over 10 days in culture. The proliferative activity of the aggregates resulted in the doubling of the aggregate cell number and a subsequent increase in the aggregate thickness, while albumin secretion levels in encapsulated aggregates was 4.5 times higher compared to non-encapsulated, control aggregates. The results reported here suggest that the ultrasound trap can provide an alternative, novel approach of hydrogel cell encapsulation and thus rapidly (within 5 min) produce in vitro models for hepatocyte functional studies (for example, toxicity studies particularly if primary hepatocytes are used) in a tissue-mimetic manner.
المشرفين على المادة: 0 (Actins)
0 (Albumins)
0 (Alginates)
0 (Biocompatible Materials)
25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
M4I0D6VV5M (Calcium Chloride)
تواريخ الأحداث: Date Created: 20080521 Date Completed: 20080829 Latest Revision: 20171116
رمز التحديث: 20231215
DOI: 10.1016/j.tiv.2008.03.014
PMID: 18490133
قاعدة البيانات: MEDLINE
الوصف
تدمد:0887-2333
DOI:10.1016/j.tiv.2008.03.014