دورية أكاديمية
Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers.
| العنوان: | Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers. |
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| المؤلفون: | Centeno DC; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany., Oliver SN, Nunes-Nesi A, Geigenberger P, Machado DN, Loureiro ME, Silva MA, Fernie AR |
| المصدر: | Physiologia plantarum [Physiol Plant] 2008 Aug; Vol. 133 (4), pp. 744-54. Date of Electronic Publication: 2008 Apr 07. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Scandinavian Society For Plant Physiology Country of Publication: Denmark NLM ID: 1256322 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1399-3054 (Electronic) Linking ISSN: 00319317 NLM ISO Abbreviation: Physiol Plant Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Copenhagen : Scandinavian Society For Plant Physiology Original Publication: Lund, Sweden [etc.] |
| مواضيع طبية MeSH: | Carbohydrate Metabolism* , Metabolic Networks and Pathways*, Plant Tubers/*metabolism , Solanum tuberosum/*metabolism, Cell Respiration ; Citric Acid Cycle ; Cytochromes/metabolism ; Glycolysis ; Immunoblotting ; Mitochondria/metabolism ; Oxidation-Reduction ; Pentose Phosphate Pathway ; Plant Tubers/enzymology ; Plants, Genetically Modified ; Saccharomyces cerevisiae/enzymology ; Solanum tuberosum/cytology ; Solanum tuberosum/genetics ; beta-Fructofuranosidase/metabolism |
| مستخلص: | In the present article we evaluate the consequence of tuber-specific expression of yeast invertase, on the pathways of carbohydrate oxidation, in potato (Solanum tuberosum L. cv. Desiree). We analysed the relative rates of glycolysis and the oxidative pentose phosphate pathway that these lines exhibited as well as the relative contributions of the cytochrome and alternative pathways of mitochondrial respiration. Enzymatic and protein abundance analysis revealed concerted upregulation of the glycolytic pathway and of specific enzymes of the tricarboxylic acid cycle and the alternative oxidase but invariant levels of enzymes of the oxidative pentose phosphate pathway and proteins of the cytochrome pathway. When taken together these experiments suggest that the overexpression of a cytosolic invertase (EC 3.2.1.26) results in a general upregulation of carbohydrate oxidation with increased flux through both the glycolytic and oxidative pentose phosphate pathways as well as the cytochrome and alternative pathways of oxidative phosphorylation. Moreover these data suggest that the upregulation of respiration is a consequence of enhanced efficient mitochondrial metabolism. |
| المشرفين على المادة: | 0 (Cytochromes) EC 3.2.1.26 (beta-Fructofuranosidase) |
| تواريخ الأحداث: | Date Created: 20080523 Date Completed: 20081029 Latest Revision: 20080820 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1111/j.1399-3054.2008.01109.x |
| PMID: | 18494735 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1399-3054 |
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| DOI: | 10.1111/j.1399-3054.2008.01109.x |