دورية أكاديمية
Evaluation of the use of UPLC-TOFMS with simultaneous [14C]-radioflow detection for drug metabolite profiling: application to propranolol metabolites in rat urine.
| العنوان: | Evaluation of the use of UPLC-TOFMS with simultaneous [14C]-radioflow detection for drug metabolite profiling: application to propranolol metabolites in rat urine. |
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| المؤلفون: | Athersuch TJ; Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics (SORA), Faculty of Medicine, Imperial College London, South Kensington, London SW7 2AZ, UK., Sison RL, Kenyon AS, Clarkson-Jones JA, Wilson ID |
| المصدر: | Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2008 Sep 10; Vol. 48 (1), pp. 151-7. Date of Electronic Publication: 2008 May 14. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 8309336 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0731-7085 (Print) Linking ISSN: 07317085 NLM ISO Abbreviation: J Pharm Biomed Anal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2006->: London : Elsevier Science Original Publication: Oxford ; New York : Pergamon Press, c1983- |
| مواضيع طبية MeSH: | Adrenergic beta-Antagonists/*pharmacokinetics , Adrenergic beta-Antagonists/*urine , Chromatography, Liquid/*methods , Mass Spectrometry/*methods , Propranolol/*pharmacokinetics , Propranolol/*urine, Adrenergic beta-Antagonists/analysis ; Animals ; Biotransformation ; Carbon Radioisotopes/metabolism ; Drug Evaluation, Preclinical/methods ; Male ; Molecular Structure ; Propranolol/analysis ; Propranolol/chemistry ; Rats ; Rats, Wistar ; Urinalysis/methods |
| مستخلص: | The non-selective beta-adrenergic receptor antagonist propranolol [1-(isopropylamino)-3-(1-naphthoxy)-2-propanol] is metabolised extensively in vivo. Enumerating and identifying the many metabolites that result from multiple biotransformations provides a considerable analytical challenge, greatly aided by efficient chromatography coupled to sensitive mass spectrometric detection. Here the use of the newly introduced high-resolution technique of "ultra performance liquid chromatography" (UPLC) linked to quadrupole time-of-flight mass spectrometry (TOFMS) with simultaneous [(14)C]-radioflow detection was applied to rapid metabolite profiling. [14C]-propranolol, dosed intraperitoneally to rat at 25 mg kg(-1) and 200 microCi kg(-1) was used as a model compound for this evaluation. Some 14 metabolites were detected in the urine by this technique including a number of conjugated metabolites such as sulphates, several isobaric glucuronides and two novel di-glucuronides. |
| المشرفين على المادة: | 0 (Adrenergic beta-Antagonists) 0 (Carbon Radioisotopes) 9Y8NXQ24VQ (Propranolol) |
| تواريخ الأحداث: | Date Created: 20080624 Date Completed: 20081126 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jpba.2008.05.003 |
| PMID: | 18571352 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0731-7085 |
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| DOI: | 10.1016/j.jpba.2008.05.003 |