دورية أكاديمية
[Determination of protease activity in blood and in microorganisms].
| العنوان: | [Determination of protease activity in blood and in microorganisms]. |
|---|---|
| المؤلفون: | Kozlov LV, Bichucher AM, Mishin AA, D'iakov VL, Leont'eva NI, Gracheva NM, Novikova LI |
| المصدر: | Biomeditsinskaia khimiia [Biomed Khim] 2008 May-Jun; Vol. 54 (3), pp. 314-21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk Country of Publication: Russia (Federation) NLM ID: 101196966 Publication Model: Print Cited Medium: Print ISSN: 2310-6972 (Print) Linking ISSN: 23106905 NLM ISO Abbreviation: Biomed Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk, 2003- |
| مواضيع طبية MeSH: | Neisseria meningitidis/*enzymology , Peptide Hydrolases/*analysis, Enzyme-Linked Immunosorbent Assay ; Humans ; Hydrogen-Ion Concentration ; Immunoglobulin G ; Pepsinogen A/blood ; Pepsinogen C/biosynthesis ; Peptide Hydrolases/blood ; Serine Endopeptidases/blood |
| مستخلص: | For determination of protease activity it is possible to use immunoglobulins. Since proteolytic products apparently do not retain substrate antigenic determinants, it is possible to use ELISA methodsfor monitoring for enzymatic process. ELISA determination of functional activity of specific IgA1-protease has been applied not only for detection of this enzyme, but also for measurement of its inhibition constants. Fixed on a micropanel IgG may be used for evaluation of total proteolytic activity. Depending on pH values, it is possible to measure activity of neutral, alkaline and acid proteases. This approach has allowed to estimate total proteolytic activity of neutral proteases of serum. Measurement of a total level of serum pepsinogene activity can have diagnostic importance in gastroenterology, due to decisive contribution of pepsinogen I to the measured activity. |
| المشرفين على المادة: | 0 (Immunoglobulin G) 61536-72-9 (Pepsinogen C) 9001-10-9 (Pepsinogen A) EC 3.4.- (Peptide Hydrolases) EC 3.4.21.- (Serine Endopeptidases) EC 3.4.21.72 (IgA-specific serine endopeptidase) |
| تواريخ الأحداث: | Date Created: 20080821 Date Completed: 20080908 Latest Revision: 20201209 |
| رمز التحديث: | 20221213 |
| PMID: | 18712087 |
| قاعدة البيانات: | MEDLINE |
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