دورية أكاديمية
Alteration of testicular steroidogenesis and histopathology of reproductive system in male rats treated with triclosan.
| العنوان: | Alteration of testicular steroidogenesis and histopathology of reproductive system in male rats treated with triclosan. |
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| المؤلفون: | Kumar V; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India., Chakraborty A, Kural MR, Roy P |
| المصدر: | Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] 2009 Apr; Vol. 27 (2), pp. 177-85. Date of Electronic Publication: 2008 Dec 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon In Cooperation With The Reproductive Toxicology Center Country of Publication: United States NLM ID: 8803591 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0890-6238 (Print) Linking ISSN: 08906238 NLM ISO Abbreviation: Reprod Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Elmsford Ny : Pergamon In Cooperation With The Reproductive Toxicology Center Original Publication: Elmsford, N.Y., U.S.A. : Pergamon Press, c1987- |
| مواضيع طبية MeSH: | Androgen Antagonists/*toxicity , Endocrine Disruptors/*toxicity , Gonadal Steroid Hormones/*blood , Spermatogenesis/*drug effects , Testis/*drug effects , Triclosan/*toxicity, 17-Hydroxysteroid Dehydrogenases/metabolism ; 3-Hydroxysteroid Dehydrogenases/metabolism ; Animals ; Body Weight/drug effects ; Cholesterol/blood ; Cholesterol Side-Chain Cleavage Enzyme/metabolism ; Dose-Response Relationship, Drug ; Follicle Stimulating Hormone/blood ; Gene Expression Regulation/drug effects ; Leydig Cells/drug effects ; Leydig Cells/metabolism ; Leydig Cells/pathology ; Luteinizing Hormone/blood ; Male ; Organ Size/drug effects ; Phosphoproteins/metabolism ; Pregnenolone/blood ; RNA, Messenger/metabolism ; Rats ; Rats, Wistar ; Receptors, Androgen/metabolism ; Steroid 17-alpha-Hydroxylase/metabolism ; Testis/metabolism ; Testis/pathology ; Testosterone/blood |
| مستخلص: | Triclosan (TCS), a chlorophenol, is widely used as a preservative in different types of commercial preparations. The reports on TCS-mediated endocrine disruption are controversial and the present study aimed to elucidate the probable mode of action of TCS as an antiandrogenic compound using a robust study design. Male albino rats, Rattus norvegicus, were treated with three doses of triclosan for a period of 60 days followed by the analysis of various biochemical parameters. RT-PCR analysis demonstrated a significant decrease in mRNA levels for testicular steroidogenic acute regulatory (StAR) protein, cytochrome P450(SCC), cytochrome P450(C17), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and androgen receptor (AR) in TCS treated rats (p<0.05). TCS also induced a perturbed translation of testicular StAR, and AR proteins as shown by Western blot analysis in treated groups of rats. A reduced level of StAR was further indicated by immunohistochemistry in testicular Leydig cells. Further, there was a significant decrease (p<0.05) in the level of serum lutenizing hormone (LH), follicle stimulating hormone (FSH), cholesterol, pregnenolone, and testosterone. In vitro assays demonstrated more than 30% decrease in testicular 3beta-HSD and 17beta-HSD enzyme activities in treated group of animals. Extensive histopathological malformations were observed in the testis and sex accessory tissues of the treated rats. Overall this study showed that TCS decreased the synthesis of androgens followed by reduced sperm production in treated male rats which could be mediated by a decreased synthesis of LH and FSH thus involving hypothalamo-pituitary-gonadal axis. |
| المشرفين على المادة: | 0 (Androgen Antagonists) 0 (Endocrine Disruptors) 0 (Gonadal Steroid Hormones) 0 (Phosphoproteins) 0 (RNA, Messenger) 0 (Receptors, Androgen) 0 (steroidogenic acute regulatory protein) 3XMK78S47O (Testosterone) 4NM5039Y5X (Triclosan) 73R90F7MQ8 (Pregnenolone) 9002-67-9 (Luteinizing Hormone) 9002-68-0 (Follicle Stimulating Hormone) 97C5T2UQ7J (Cholesterol) EC 1.1.- (17-Hydroxysteroid Dehydrogenases) EC 1.1.- (3-Hydroxysteroid Dehydrogenases) EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase) EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme) |
| تواريخ الأحداث: | Date Created: 20090103 Date Completed: 20090602 Latest Revision: 20161125 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.reprotox.2008.12.002 |
| PMID: | 19118620 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0890-6238 |
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| DOI: | 10.1016/j.reprotox.2008.12.002 |