دورية أكاديمية
[Cloning and expression of human Runx3 gene obtained from human peripheral blood CD8(+) T lymphocyte].
| العنوان: | [Cloning and expression of human Runx3 gene obtained from human peripheral blood CD8(+) T lymphocyte]. |
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| المؤلفون: | Li YZ; Department of Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Zhenjiang, China. lyz-0115@163.com, Wang SJ, Shi Y, Dai XL, Peng SF, Su ZL, Shao QX, Xu HX |
| المصدر: | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2009 Feb; Vol. 25 (2), pp. 107-10. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Xi bao yu fen zi mian yi xue za zhi bian ji bu Country of Publication: China NLM ID: 101139110 Publication Model: Print Cited Medium: Print ISSN: 1007-8738 (Print) Linking ISSN: 10078738 NLM ISO Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Xiʹan : Xi bao yu fen zi mian yi xue za zhi bian ji bu |
| مواضيع طبية MeSH: | CD8-Positive T-Lymphocytes/*metabolism , Core Binding Factor Alpha 3 Subunit/*metabolism, Amino Acid Sequence ; Base Sequence ; Blotting, Western ; Cells, Cultured ; Cloning, Molecular ; Core Binding Factor Alpha 3 Subunit/chemistry ; Core Binding Factor Alpha 3 Subunit/genetics ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Humans ; Molecular Sequence Data ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment |
| مستخلص: | Aim: Runx3, a type of Runt family member, plays an important role in immune regulation. In this study, we cloned and analyzed the cDNA encoding human Runx3 from T lymphocyte, expressed Runx3 protein in E.coli system, and studied the relation between Runx3 and some immune disorders or tumors. Methods: The CD8(+) T were isolated from human peripheral blood with MACS, Runx3 cDNA was amplified by RT-PCR and cloned into pMD19-T vector, and recombinant was transformed into competent cells DH5alpha and recombinant sequencing were performed. The identical was subcloned into pQE30 vector and expressed in E.coli M15. The fusion protein was identified by Western blot. Results: The 1,248 bp fragment amplified by RT-PCR was the same as the anticipated one in size and encodes 415 amino acids. Runx3 protein was gained. Conclusion: Human Runx3 gene was cloned and expressed in E.coli system successfully, which brought a foundation for further research on its biological function. |
| المشرفين على المادة: | 0 (Core Binding Factor Alpha 3 Subunit) 0 (Runx3 protein, human) |
| تواريخ الأحداث: | Date Created: 20090129 Date Completed: 20100408 Latest Revision: 20201209 |
| رمز التحديث: | 20231215 |
| PMID: | 19174005 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1007-8738 |
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