دورية أكاديمية
Determining the time needed for the vortex method for preparing solvent-free MALDI samples of low molecular mass polymers.
| العنوان: | Determining the time needed for the vortex method for preparing solvent-free MALDI samples of low molecular mass polymers. |
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| المؤلفون: | Hanton SD; Air Products and Chemicals, Inc., Allentown, Pennsylvania 18195, USA. HantonSD@airproducts.com, Stets JR |
| المصدر: | Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2009 Jun; Vol. 20 (6), pp. 1115-8. Date of Electronic Publication: 2009 Feb 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: ACS Publications Country of Publication: United States NLM ID: 9010412 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1123 (Electronic) Linking ISSN: 10440305 NLM ISO Abbreviation: J Am Soc Mass Spectrom Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : Washington, DC : ACS Publications Original Publication: New York, NY : Elsevier, c1990- |
| مستخلص: | Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize many different materials, including synthetic polymers. MALDI mass spectral data is used to determine the polymer average molecular weights, repeat units, and end groups. The development of the vortex method of solvent-free sample preparation showed that remarkably short mixing times could prepare samples that yielded high quality MALDI mass spectra. In this paper, we use microscopy images and MALDI mass spectra to evaluate the mixing time required by the vortex method to produce mass spectra for low molecular mass polymer samples. Our results show that mixing times of as little as 10 s can generate homogeneous thin films that produce high quality mass spectra with S/N approximately 100. In addition, ultrashort mixing times of only 2 s still produce samples with mostly smooth morphology and mass spectra with S/N approximately 10. |
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| تواريخ الأحداث: | Date Created: 20090324 Date Completed: 20090903 Latest Revision: 20211020 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jasms.2009.02.007 |
| PMID: | 19303322 |
| قاعدة البيانات: | MEDLINE |
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