دورية أكاديمية
Combined use of GC-TOF MS and UHPLC-(Q)TOF MS to investigate the presence of nontarget pollutants and their metabolites in a case of honeybee poisoning.
| العنوان: | Combined use of GC-TOF MS and UHPLC-(Q)TOF MS to investigate the presence of nontarget pollutants and their metabolites in a case of honeybee poisoning. |
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| المؤلفون: | Portolés T; Research Institute for Pesticides and Water, University Jaume I, E-12071 Castellón, Spain., Ibáñez M, Sancho JV, López FJ, Hernández F |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2009 May 27; Vol. 57 (10), pp. 4079-90. Date of Electronic Publication: 2009 Apr 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Bees/*chemistry , Bees/*drug effects , Chromatography, High Pressure Liquid/*methods , Gas Chromatography-Mass Spectrometry/*methods , Insecticides/*analysis , Insecticides/*poisoning , Mass Spectrometry/*methods, Animals ; Coumaphos/analysis ; Flowers/chemistry ; Plant Leaves/chemistry ; Prunus/chemistry |
| مستخلص: | The combined use of gas chromatography (GC) and ultrahigh-pressure liquid chromatography (UHPLC), both coupled to time-of-flight mass spectrometry (TOF MS), has been explored in this work for the investigation of several cases of honeybee poisoning. The procedure applied involves a previous extraction with acetone followed by liquid-liquid extraction with dichloromethane. Both techniques, GC-TOF MS and UHPLC-(Q)TOF MS, have been applied to discover the presence of compounds that might be responsible of honeybee deaths. The application of a nontarget methodological approach to a first case of poisoning allowed the detection of the insecticide coumaphos at high concentration levels in the samples. The presence of possible metabolites of this organophosphorus insecticide was investigated by using both techniques. UHPLC-(Q)TOF MS showed its higher applicability in this case, as most of the metabolites were more polar than the parent compound. Four metabolites were identified by UHPLC-(Q)TOF MS, whereas only two of them were found by GC-TOF MS. The developed methodology was applied to other subsequent poisoning cases in which insecticides such as coumaphos, thiamethoxam, pyriproxyfen, and chlorfenvinphos were identified by both techniques, whereas GC-TOF MS also allowed the detection of fenitrothion and methiocarb. In all positive cases, the confirmation of the presence of the compound detected was feasible by means of accurate mass measurements of up to five ions together with their ion ratio evaluation. |
| المشرفين على المادة: | 0 (Insecticides) L08SZ5Z5JC (Coumaphos) |
| تواريخ الأحداث: | Date Created: 20090423 Date Completed: 20110524 Latest Revision: 20131121 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/jf900099u |
| PMID: | 19382786 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5118 |
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| DOI: | 10.1021/jf900099u |