دورية أكاديمية
أعرض في EDS

Snap-frozen brain tissue sections stored with desiccant at ambient laboratory conditions without chemical fixation are resistant to degradation for a minimum of 6 months.

التفاصيل البيبلوغرافية
العنوان: Snap-frozen brain tissue sections stored with desiccant at ambient laboratory conditions without chemical fixation are resistant to degradation for a minimum of 6 months.
المؤلفون: Sadler TR; Department of Pathology, USC Keck School of Medicine, Los Angeles, CA 90089-9092, USA. tsadler@usc.edu, Khodavirdi AC, Hinton DR, Holschneider DP
المصدر: Applied immunohistochemistry & molecular morphology : AIMM [Appl Immunohistochem Mol Morphol] 2009 Mar; Vol. 17 (2), pp. 165-71.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 100888796 Publication Model: Print Cited Medium: Internet ISSN: 1533-4058 (Electronic) Linking ISSN: 15334058 NLM ISO Abbreviation: Appl Immunohistochem Mol Morphol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Hagerstown, MD : Lippincott Williams & Wilkins, c1999-
مواضيع طبية MeSH: Brain Chemistry*, Cryopreservation/*methods , Frozen Sections/*methods, Animals ; Chemical Fractionation ; Mice ; Proteins/chemistry ; Rats ; Time Factors
مستخلص: Cryosectioned tissues from snap-frozen samples offer the advantage of preserving proteins at the cellular and subcellular levels and maintaining overall cell integrity in the tissue of interest without the use of chemical fixatives. To prevent specific or nonspecific degradation of proteins by autolytic and/or proteolytic processes, it is common practice to immediately store frozen tissue sections obtained from a cryostat under cryogenic conditions, for example -80 degrees C. Our laboratory recently challenged this widely held belief by extracting proteins from brain tissue samples that were archived for 1 day, 1 week, 1 month, and 6 months at various storage conditions (frozen, ambient, or desiccated) without the use of chemical fixatives. Our results from immunofluorescent stains, immunoperoxidase stains, silver stains, and Western blot analyses demonstrated that snap-frozen, heat-dried tissue sections stored and desiccated at ambient laboratory conditions are comparable to frozen samples stored up to 6 months.
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معلومات مُعتمدة: R24 AT002681 United States AT NCCIH NIH HHS; 5R24AT002681 United States AT NCCIH NIH HHS; R24 AT002681-05 United States AT NCCIH NIH HHS; P30 EY003040 United States EY NEI NIH HHS; 5P30EY003040 United States EY NEI NIH HHS; P30 EY003040-259003 United States EY NEI NIH HHS
المشرفين على المادة: 0 (Proteins)
تواريخ الأحداث: Date Created: 20090613 Date Completed: 20090818 Latest Revision: 20211020
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC2742633
DOI: 10.1097/PAI.0b013e3181853001
PMID: 19521279
قاعدة البيانات: MEDLINE
الوصف
تدمد:1533-4058
DOI:10.1097/PAI.0b013e3181853001