دورية أكاديمية
Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: expression profile of genes in the nitric oxide pathway of penile tissue of mice.
| العنوان: | Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: expression profile of genes in the nitric oxide pathway of penile tissue of mice. |
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| المؤلفون: | Villanova FE; Laboratory of Medical Investigation-LIM55, University of São Paulo, São Paulo, Brazil. fvillanova@gmail.com, Andrade E, Leal E, Andrade PM, Borra RC, Troncone LR, Magalhães L, Leite KR, Paranhos M, Claro J, Srougi M |
| المصدر: | Toxicon : official journal of the International Society on Toxinology [Toxicon] 2009 Nov; Vol. 54 (6), pp. 793-801. Date of Electronic Publication: 2009 Jun 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1962- |
| مواضيع طبية MeSH: | Gene Expression Profiling*, Nitric Oxide/*metabolism , Penile Erection/*drug effects , Peptides/*pharmacology , Spider Venoms/*pharmacology, Animals ; Base Sequence ; DNA Primers ; Male ; Mice ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction |
| مستخلص: | The peptides Tx2-5 and Tx2-6, isolated from the whole venom of "armed-spider"Phoneutria nigriventer venom, are directly linked with the induction of persistent and painful erection in the penis of mammals. The erection induced by Tx2-6 has been associated with the activation of nitric oxide synthases. There is a scarcity of studies focusing on the outcome of Tx2-6 at the molecular level, by this reason we evaluated the gene profile activity of this toxin at the nitric oxide (NO) pathway. After microarray analyses on cavernous tissue of mice inoculated with Tx2-6 we found that only 10.4% (10/96) of these genes were differentially expressed, showing a limited effect of the toxin on the NO pathway. We found the genes sparc, ednrb, junb, cdkn1a, bcl2, ccl5, abcc1 over-expressed and the genes sod1, s100a10 and fth1 under-expressed after inoculation of Tx2-6. The differential expressions of sparc and ednrb genes were further confirmed using real-time PCR. Interestingly, ednrb activates the L-arginine/NO/cGMP pathway that is involved in the relaxation of the cavernous body. Therefore the priapism induced by Tx2-6 is a consequence of a highly specific interference of this neurotoxin with the NO pathway. |
| المشرفين على المادة: | 0 (DNA Primers) 0 (Peptides) 0 (Spider Venoms) 0 (Tx2-6 protein, Phoneutria nigriventer) 31C4KY9ESH (Nitric Oxide) |
| تواريخ الأحداث: | Date Created: 20090616 Date Completed: 20091221 Latest Revision: 20131121 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.toxicon.2009.06.006 |
| PMID: | 19524607 |
| قاعدة البيانات: | MEDLINE |
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