دورية أكاديمية
Development and validation of a simple method for routine analysis of ractopamine hydrochloride in raw material and feed additives by HPLC.
| العنوان: | Development and validation of a simple method for routine analysis of ractopamine hydrochloride in raw material and feed additives by HPLC. |
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| المؤلفون: | Freire EF; University of São Paulo, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto, Brazil., Borges KB, Tanimoto H, Nogueira RT, Bertolini LC, de Gaitani CM |
| المصدر: | Journal of AOAC International [J AOAC Int] 2009 May-Jun; Vol. 92 (3), pp. 757-64. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9215446 Publication Model: Print Cited Medium: Print ISSN: 1060-3271 (Print) Linking ISSN: 10603271 NLM ISO Abbreviation: J AOAC Int Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : Oxford : Oxford University Press Original Publication: Arlington, VA : AOAC International, c1992- |
| مواضيع طبية MeSH: | Chromatography, High Pressure Liquid/*methods , Food Additives/*analysis , Phenethylamines/*analysis, Drug Stability |
| مستخلص: | A simple method was optimized and validated for determination of ractopamine hydrochloride (RAC) in raw material and feed additives by HPLC for use in quality control in veterinary industries. The best-optimized conditions were a C8 column (250 x 4.6 mm id, 5.0 microm particle size) at room temperature with acetonitrile-100 mM sodium acetate buffer (pH 5.0; 75 + 25, v/v) mobile phase at a flow rate of 1.0 mL/min and UV detection at 275 nm. With these conditions, the retention time of RAC was around 5.2 min, and standard curves were linear in the concentration range of 160-240 microg/mL (correlation coefficient > or = 0.999). Validation parameters, such as selectivity, linearity, limit of detection (ranged from 1.60 to 2.05 microg/mL), limit of quantification (ranged from 4.26 to 6.84 microg/mL), precision (relative standard deviation < or = 1.87%), accuracy (ranged from 96.97 to 100.54%), and robustness, gave results within acceptable ranges. Therefore, the developed method can be successfully applied for the routine quality control analysis of raw material and feed additives. |
| المشرفين على المادة: | 0 (Food Additives) 0 (Phenethylamines) 57370OZ3P1 (ractopamine) |
| تواريخ الأحداث: | Date Created: 20090721 Date Completed: 20090811 Latest Revision: 20191210 |
| رمز التحديث: | 20240829 |
| PMID: | 19610364 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1060-3271 |
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