دورية أكاديمية
Structural analysis of the short length ribosomal DNA variant from Pisum sativum L. cv. Alaska.
| العنوان: | Structural analysis of the short length ribosomal DNA variant from Pisum sativum L. cv. Alaska. |
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| المؤلفون: | Piller KJ; Department of Biological Sciences, University of Illinois, Chicago 60680., Baerson SR, Polans NO, Kaufman LS |
| المصدر: | Nucleic acids research [Nucleic Acids Res] 1990 Jun 11; Vol. 18 (11), pp. 3135-45. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Print ISSN: 0305-1048 (Print) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1992- : Oxford : Oxford University Press Original Publication: London, Information Retrieval ltd. |
| مواضيع طبية MeSH: | Chromosomes, Human, Pair 4* , Plants, Medicinal*, DNA, Ribosomal/*genetics , Fabaceae/*genetics , RNA, Ribosomal/*genetics , RNA, Ribosomal, 18S/*genetics, Base Sequence ; Cloning, Molecular ; Genetic Linkage ; Humans ; Methylation ; Molecular Sequence Data ; Nucleic Acid Conformation ; Polymorphism, Restriction Fragment Length ; RNA Polymerase I/metabolism ; Repetitive Sequences, Nucleic Acid ; Restriction Mapping ; Sequence Homology, Nucleic Acid |
| مستخلص: | The genomic clone, RRNpss1, representing the short ribosomal DNA length variant in Pisum sativum L. cv. Alaska, has been isolated and the 2859 bp intergenic spacer, along with the 25S rRNA 3' border and 18S rRNA 5' border, has been sequenced. The intergenic spacer contains nine tandem repeats, approximately 180 bp in length, which show greater than 80% sequence homology to each other. The RNA polymerase I transcription start site and a processing site, located 776 bp and 536 bp upstream of the 5' end of 18S rRNA, respectively, have been determined using S1 analysis. The region surrounding the +1 site shows strong homology between the positions -6 to +10 to the rDNA sites of initiation in radish, maize, and wheat. The sequence CATGCAAA is located 19 bp upstream of the site of initiation, and appears once within each subrepeat and twice more between the end of the subrepeat array and the site of initiation. A previously characterized HpaII site which shows developmental regulation of methylation is located 31 bp downstream of the site of initiation. Using RFLP linkage analysis, the short rDNA length variant of cv. Alaska is assigned to Chromosome 4 where it is genetically independent of the long rDNA length variant which is putatively assigned to Chromosome 7. |
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| معلومات مُعتمدة: | BRSG-S07-RR07176 United States RR NCRR NIH HHS |
| سلسلة جزيئية: | GENBANK X52575 |
| المشرفين على المادة: | 0 (DNA, Ribosomal) 0 (RNA, Ribosomal) 0 (RNA, Ribosomal, 18S) EC 2.7.7.6 (RNA Polymerase I) |
| تواريخ الأحداث: | Date Created: 19900611 Date Completed: 19900725 Latest Revision: 20190501 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC330916 |
| DOI: | 10.1093/nar/18.11.3135 |
| PMID: | 1972558 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0305-1048 |
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| DOI: | 10.1093/nar/18.11.3135 |