دورية أكاديمية

Carbonic anhydrase II-based metal ion sensing: Advances and new perspectives.

التفاصيل البيبلوغرافية
العنوان: Carbonic anhydrase II-based metal ion sensing: Advances and new perspectives.
المؤلفون: Hurst TK; Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA., Wang D, Thompson RB, Fierke CA
المصدر: Biochimica et biophysica acta [Biochim Biophys Acta] 2010 Feb; Vol. 1804 (2), pp. 393-403. Date of Electronic Publication: 2009 Oct 08.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Review
اللغة: English
بيانات الدورية: Publisher: Elsevier Pub. Co Country of Publication: Netherlands NLM ID: 0217513 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0006-3002 (Print) Linking ISSN: 00063002 NLM ISO Abbreviation: Biochim Biophys Acta Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Pub. Co.
مواضيع طبية MeSH: Carbonic Anhydrase II/*chemistry , Zinc/*chemistry, Carbonic Anhydrase II/metabolism ; Humans ; Zinc/metabolism
مستخلص: Carbonic anhydrases are archetypical zinc metalloenzymes and as such, they have been developed as the recognition element of a family of fluorescent indicators (sensors) to detect metal ions, particularly Zn(2+) and Cu(2+). Subtle modification of the structure of human carbonic anhydrase II isozyme (CAII) alters the selectivity, sensitivity, and response time for these sensors. Sensors using CAII variants coupled with zinc-dependent fluorescent ligands demonstrate picomolar sensitivity, unmatched selectivity, ratiometric fluorescence signal, and near diffusion-controlled response times. Recently, these sensors have been applied to measuring the readily exchangeable concentrations of zinc in the cytosol and nucleus of mammalian tissue culture cells and concentrations of free Cu(2+) in seawater.
(Copyright 2009 Elsevier B.V. All rights reserved.)
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معلومات مُعتمدة: GM40602 United States GM NIGMS NIH HHS; R01 EB003924-05 United States EB NIBIB NIH HHS; R01 EB003924 United States EB NIBIB NIH HHS; 1 RO1 EB03924 United States EB NIBIB NIH HHS; R01 GM040602 United States GM NIGMS NIH HHS; R01 GM040602-21 United States GM NIGMS NIH HHS
المشرفين على المادة: EC 4.2.1.- (Carbonic Anhydrase II)
J41CSQ7QDS (Zinc)
تواريخ الأحداث: Date Created: 20091013 Date Completed: 20100401 Latest Revision: 20211020
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC2818387
DOI: 10.1016/j.bbapap.2009.09.031
PMID: 19818877
قاعدة البيانات: MEDLINE