دورية أكاديمية
أعرض في EDS

Protective effects of acetyl-L-carnitine on cisplatin cytotoxicity and oxidative stress in neuroblastoma.

التفاصيل البيبلوغرافية
العنوان: Protective effects of acetyl-L-carnitine on cisplatin cytotoxicity and oxidative stress in neuroblastoma.
المؤلفون: Altun ZS; Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Inciralti, 35340, Izmir, Turkey. zekiye.altun@deu.edu.tr, Güneş D, Aktaş S, Erbayraktar Z, Olgun N
المصدر: Neurochemical research [Neurochem Res] 2010 Mar; Vol. 35 (3), pp. 437-43. Date of Electronic Publication: 2009 Oct 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 7613461 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6903 (Electronic) Linking ISSN: 03643190 NLM ISO Abbreviation: Neurochem Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1999- : New York, NY : Kluwer Academic/Plenum Publishers
Original Publication: New York, Plenum Press
مواضيع طبية MeSH: Neuroprotective Agents*, Acetylcarnitine/*pharmacology , Antineoplastic Agents/*toxicity , Brain Neoplasms/*metabolism , Cisplatin/*antagonists & inhibitors , Cisplatin/*toxicity , Neuroblastoma/*metabolism , Nootropic Agents/*pharmacology , Oxidative Stress/*drug effects, Apoptosis/drug effects ; Brain Neoplasms/pathology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Coloring Agents ; Enzyme-Linked Immunosorbent Assay ; Glutathione/metabolism ; Humans ; L-Lactate Dehydrogenase/metabolism ; Lipid Peroxidation/drug effects ; Neuroblastoma/pathology ; Proto-Oncogene Proteins c-myc/metabolism ; Tetrazolium Salts ; Thiazoles
مستخلص: The most widely used platinum-derived drug is cisplatin in neuroblastoma (NB) chemotherapy, which is severely neurotoxic. Acetyl-L-Carnitine (ALC) is a natural occurring compound with a neuroprotective activity in several experimental paradigms. The aim of this study was to determine the effects of ALC on cisplatin induced cytotoxicity and oxidative stress in NB cells. SH-SY5Y (N-Myc negative) and KELLY (N-Myc positive) human NB cell lines were used. Cisplatin induced apoptosis was assessed by using a Cell Death Detection ELISA(PLUS) kit. Lipid peroxidation levels were determined by HPLC analysis. Glutathione levels were determined spectrophotometrically. ALC was used prophylactic or after cisplatin application. The level of cisplatin doses were determined in both type of NB cells at which 50% cell death occurred along with synchronized apoptosis induced. Prophylactic 10 and 50 micromol of ALC concentrations were decreased cisplatin induced lipid peroxidation compared to controls that normally exhibited apoptosis especially in SH-SY5Y cells. Cisplatin caused oxidative stress through decreasing glutathione levels in both cell types. ALC were effectively inhibited the increase in cisplatin induced oxidized glutathione and lipid peroxidation formation in NB cells. We suggested that prophylactic ALC would be a useful agent for cisplatin induced toxicity in NB cells.
التعليقات: Erratum in: Neurochem Res. 2010 Apr;35(4):679. Erbayrktar, Zübeyde [corrected to Erbayraktar, Zübeyde].
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المشرفين على المادة: 0 (Antineoplastic Agents)
0 (Coloring Agents)
0 (Neuroprotective Agents)
0 (Nootropic Agents)
0 (Proto-Oncogene Proteins c-myc)
0 (Tetrazolium Salts)
0 (Thiazoles)
6DH1W9VH8Q (Acetylcarnitine)
EC 1.1.1.27 (L-Lactate Dehydrogenase)
EUY85H477I (thiazolyl blue)
GAN16C9B8O (Glutathione)
Q20Q21Q62J (Cisplatin)
تواريخ الأحداث: Date Created: 20091024 Date Completed: 20100430 Latest Revision: 20211020
رمز التحديث: 20221213
DOI: 10.1007/s11064-009-0076-8
PMID: 19851866
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-6903
DOI:10.1007/s11064-009-0076-8