دورية أكاديمية
[Dynamic changes of dopamine and its metabolite levels in the rat striatum after cerebral ischemia-reperfusion and electroacupuncture].
| العنوان: | [Dynamic changes of dopamine and its metabolite levels in the rat striatum after cerebral ischemia-reperfusion and electroacupuncture]. |
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| المؤلفون: | Xu MS; Shanghai Research Institute of Acu-moxibustion and Meridians, Shanghai 200030, China. mingshuxu@yahoo.com.cn, Fang C, Xu J, Zhang GF, Ge LB |
| المصدر: | Zhen ci yan jiu = Acupuncture research [Zhen Ci Yan Jiu] 2009 Aug; Vol. 34 (4), pp. 230-5. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Institute Of Acupuncture And Moxibustion Country of Publication: China NLM ID: 8507710 Publication Model: Print Cited Medium: Print ISSN: 1000-0607 (Print) Linking ISSN: 10000607 NLM ISO Abbreviation: Zhen Ci Yan Jiu Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Institute Of Acupuncture And Moxibustion Original Publication: Beijing, China : Published and distributed by World Books, [1980- |
| مواضيع طبية MeSH: | Electroacupuncture*, Brain Ischemia/*metabolism , Dopamine/*metabolism , Neostriatum/*metabolism , Reperfusion Injury/*metabolism , Reperfusion Injury/*therapy, 3,4-Dihydroxyphenylacetic Acid/metabolism ; Animals ; Brain Ischemia/pathology ; Extracellular Fluid ; Homovanillic Acid/metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results |
| مستخلص: | Objective: To explore the involvement of dopamine (DA) and its metabolites in the extracellular fluid of the striatum on the resisting action of electroacupuncture (EA) for acute cerebral ischemia-reperfusion (CI/R) injury. Methods: A total of 50 SD male rats were randomly divided into normal control, sham-operation (sham), sham + EA, CI/R model, CI/R + EA groups. CI/R model was induced by right middle cerebral artery occlusion (MCAO) for 90 min and reperfusion for 225 min. EA (2 Hz, 3 mA, continuous waves) was applied to "Fengchi" (GB 20) for 20 min, with a 10 min rest, and given once again fo 20 min. The concentrations of DA and its metabolites [3,4- dihydroxy-phenyl acetic acid (DOPAC) and homovanillic acid (HVA)] in the extracellular fluid of the striatum were determined by microdialysis and high performance liquid chromatography (HPLC). Results: The extracellular levels of DA, DOPAC and HVA in normal group, sham group and sham+ EA group had no significant changes during the whole study. The extracellular level of DA peaked 15-45 min after CI and 0-30 min after CI/R (P < 0.05). A rising trend was also observed 120 min following CI/R. DOPAC level peaked 15 min and 75 min after CI and 75 min after CI/R. After EA, DA level was significantly lower in EA group than that in model group 90 min after CI/R (P < 0.05), and no peak was observed again. After EA, DOPAC level maintained its basic level, and no peak was found after CI/R. The changing trend of HVA was similar to that of DOPAC, and the extracellular HVA level in EA + model group was significantly lower than that in model group 75 min after CI/R. Conclusion: EA can decrease the accumulation of DA and its metabolites in the extracellular fluid of the striatum, which may contribute to its effect in preventing the brain from CI/R injury. |
| المشرفين على المادة: | 102-32-9 (3,4-Dihydroxyphenylacetic Acid) VTD58H1Z2X (Dopamine) X77S6GMS36 (Homovanillic Acid) |
| تواريخ الأحداث: | Date Created: 20091118 Date Completed: 20100521 Latest Revision: 20161020 |
| رمز التحديث: | 20240829 |
| PMID: | 19916285 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-0607 |
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