التفاصيل البيبلوغرافية
| العنوان: |
[Comparison of nested-PCR with blood smear examination in detection of Ehrlichia canis and Anaplasma platys in dogs]. |
| عنوان ترانسليتريتد: |
Comparação de nested-PCR com o diagnóstico direto na detecçáo de Ehrlichia canis e Anaplasma platys em cães. |
| المؤلفون: |
Ramos CA; Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco-UFRPE, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, CEP 52171-900, Recife-PE, Brazil. carlosanramos@yahoo.com.br, Ramos RA, Araújo FR, Guedes DS Jr, Souza II, Ono TM, Vieira AS, Pimentel DS, Rosas EO, Faustino MA, Alves LC |
| المصدر: |
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria [Rev Bras Parasitol Vet] 2009 Dec; Vol. 18 Suppl 1, pp. 58-62. |
| نوع المنشور: |
Comparative Study; English Abstract; Journal Article |
| اللغة: |
Portuguese |
| بيانات الدورية: |
Publisher: Colégio Brasileiro de Parasitologia Veterinária Country of Publication: Brazil NLM ID: 9440482 Publication Model: Print Cited Medium: Print ISSN: 0103-846X (Print) Linking ISSN: 0103846X NLM ISO Abbreviation: Rev Bras Parasitol Vet Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: São Paulo, Brasil : Colégio Brasileiro de Parasitologia Veterinária, [1992]- |
| مواضيع طبية MeSH: |
Polymerase Chain Reaction*, Anaplasma/*isolation & purification , Anaplasmosis/*blood , Anaplasmosis/*diagnosis , Ehrlichia canis/*isolation & purification , Ehrlichiosis/*blood , Ehrlichiosis/*diagnosis, Animals ; Dogs ; Female ; Male |
| مستخلص: |
The clinical signs of Ehrlichia canis and Anaplasma platys infection are similar, and the diagnosis of these pathogens made by stained blood smears is poor due sensibility and specificity. On the other hand, the molecular diagnosis is highly sensitive and specific and nested-PCR have been optimized for accurate diagnosis these pathogens in dogs. At the veterinary teaching hospital, whole-blood samples with EDTA were obtained from 100 dogs and smears were made from blood samples for evaluation for intracellular parasites. For each sample, DNA was extracted and submitted to nPCR analysis for detection of E. canis and A. platys. The results of stained blood smears showed 9% of the animals were positive for E. canis and 21% for A. platys. Regarding of nPCR analysis, 57 and 55% of dogs were positive for E. canis and A. platys respectively. As compared to a nested PCR, the stained blood smears revealed false-negative results for both E. canis and A. platys. The results indicate that the nPCR is highly sensitive and specific for detection of both pathogens and the molecular diagnosis could be more useful at veterinary hospital. |
| تواريخ الأحداث: |
Date Created: 20091231 Date Completed: 20100706 Latest Revision: 20191111 |
| رمز التحديث: |
20221213 |
| DOI: |
10.4322/rbpv.018e1011 |
| PMID: |
20040193 |
| قاعدة البيانات: |
MEDLINE |
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