دورية أكاديمية
أعرض في EDS

Genetic analysis of the cause of endometrial osseous metaplasia.

التفاصيل البيبلوغرافية
العنوان: Genetic analysis of the cause of endometrial osseous metaplasia.
المؤلفون: Parente RCM; From the Departments of Gynecology Universidade Federal de São Paulo, São Paulo, Brazil; Universidade Do Estado Do Rio de Janeiro, Rio de Janeiro, Brazil; Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Instituto de Biofísica Carlos Chagas Filha, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Patriarca MT, de Moura Neto RS, de Oliveira MAP, Lasmar RB, de Holanda Mendes P, de Sá PG, Cardeman L, Silva R, de Freitas V
المصدر: Obstetrics and gynecology [Obstet Gynecol] 2009 Nov; Vol. 114 (5), pp. 1103-1108.
نوع المنشور: Case Reports; Journal Article
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0401101 Publication Model: Print Cited Medium: Internet ISSN: 1873-233X (Electronic) Linking ISSN: 00297844 NLM ISO Abbreviation: Obstet Gynecol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2004- : Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: New York.
مواضيع طبية MeSH: Bone and Bones/*pathology , Endometrium/*pathology , Uterine Diseases/*genetics , Uterine Diseases/*pathology, Adult ; Aged ; DNA/analysis ; DNA/blood ; Female ; Humans ; Metaplasia ; Middle Aged ; Pregnancy
مستخلص: Objective: To analyze solitary bone fragments from the uterine cavity through DNA genotyping, thus elucidating whether they originate from metaplasia, from previous abortion, or both.
Methods: We conducted a case series study on 14 patients, of whom eight yielded bone DNA. The patients selected had histopathologic diagnoses of bone fragments inside the uterine cavity or previously removed samples available for analysis. We extracted DNA from blood and bone fragments. To identify the bone tissue origin, these materials were genotyped using polymerase chain reactions for DNA loci. Six mini short tandem repeat loci frequently used for human tissue identification were analyzed using automated sequencing.
Results: Among these eight patients, blood and tissue samples from the same individual produced exactly the same pair of alleles for all six loci. This indicated that the DNA profile was completely the same for the bone samples and the mother's blood (95% confidence interval 63-100%), thus confirming that the DNA had the same origin and that these were cases of metaplasia.
Conclusion: In all of the eight cases, bone formation was caused by osseous metaplasia, because the DNA in the bone fragment and in the patient's blood was identical. Although all of the women had histories of previous abortion, no difference in DNA was detected in the bone tissue in any of the cases, as would be expected if abortion had occurred. This result was completely unexpected, differing greatly from what the literature suggests.
Level of Evidence: III.
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المشرفين على المادة: 9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20100220 Date Completed: 20100315 Latest Revision: 20210107
رمز التحديث: 20240628
DOI: 10.1097/AOG.0b013e3181bd198c
PMID: 20168113
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-233X
DOI:10.1097/AOG.0b013e3181bd198c