دورية أكاديمية
أعرض في EDS

A real-time RT-PCR for detection of clade 1 and 2 H5N1 influenza A virus using locked nucleic acid (LNA) TaqMan probes.

التفاصيل البيبلوغرافية
العنوان: A real-time RT-PCR for detection of clade 1 and 2 H5N1 influenza A virus using locked nucleic acid (LNA) TaqMan probes.
المؤلفون: Thanh TT; Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Dist 05, Ho Chi Minh City, Viet Nam. thanhtt@oucru.org, Pawestri HA, Ngoc NM, Hien VM, Syahrial H, Trung NV, van Doorn RH, Wertheim HF, Chau NV, Ha do Q, Farrar JJ, Hien TT, Sedyaningsih ER, de Jong MD
المصدر: Virology journal [Virol J] 2010 Feb 22; Vol. 7, pp. 46. Date of Electronic Publication: 2010 Feb 22.
نوع المنشور: Comparative Study; Evaluation Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101231645 Publication Model: Electronic Cited Medium: Internet ISSN: 1743-422X (Electronic) Linking ISSN: 1743422X NLM ISO Abbreviation: Virol J Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central, 2004-
مواضيع طبية MeSH: Influenza A Virus, H5N1 Subtype/*classification , Influenza A Virus, H5N1 Subtype/*isolation & purification , Influenza, Human/*diagnosis , Oligonucleotide Probes/*genetics , Oligonucleotides/*genetics , Reverse Transcriptase Polymerase Chain Reaction/*methods, Conserved Sequence ; Hemagglutinin Glycoproteins, Influenza Virus/genetics ; Humans ; Influenza A Virus, H5N1 Subtype/genetics ; Influenza, Human/virology ; Sensitivity and Specificity ; Vietnam
مستخلص: Background: The emergence and co-circulation of two different clades (clade 1 and 2) of H5N1 influenza viruses in Vietnam necessitates the availability of a diagnostic assay that can detect both variants.
Results: We developed a single real-time RT-PCR assay for detection of both clades of H5N1 viruses, directly from clinical specimens, using locked nucleic acid TaqMan probes. Primers and probe used in this assay were designed based on a highly conserved region in the HA gene of H5N1 viruses. The analytical sensitivity of the assay was < 0.5 PFU and 10-100 ssDNA plasmid copies. A total of 106 clinical samples (58 from patients infected with clade 1, 2.1 or 2.3 H5N1 viruses and 48 from uninfected or seasonal influenza A virus-infected individuals) were tested by the assay. The assay showed 97% concordance with initial diagnostics for H5 influenza virus infection with a specificity of 100%.
Conclusions: This assay is a useful tool for diagnosis of H5N1 virus infections in regions where different genetic clades are co-circulating.
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المشرفين على المادة: 0 (Hemagglutinin Glycoproteins, Influenza Virus)
0 (Oligonucleotide Probes)
0 (Oligonucleotides)
0 (hemagglutinin, avian influenza A virus)
0 (locked nucleic acid)
تواريخ الأحداث: Date Created: 20100223 Date Completed: 20100421 Latest Revision: 20211020
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC2838857
DOI: 10.1186/1743-422X-7-46
PMID: 20170549
قاعدة البيانات: MEDLINE
الوصف
تدمد:1743-422X
DOI:10.1186/1743-422X-7-46