دورية أكاديمية
[Construction and identification of a new type of recombinant adenovirus containing S0.7 gene of Hantavirus].
| العنوان: | [Construction and identification of a new type of recombinant adenovirus containing S0.7 gene of Hantavirus]. |
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| المؤلفون: | Li K; Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China., Li PY, Bai WT, Hu G, Wu XA, Xu ZK, Zhang FL |
| المصدر: | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2010 May; Vol. 26 (5), pp. 420-2. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Xi bao yu fen zi mian yi xue za zhi bian ji bu Country of Publication: China NLM ID: 101139110 Publication Model: Print Cited Medium: Print ISSN: 1007-8738 (Print) Linking ISSN: 10078738 NLM ISO Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Xiʹan : Xi bao yu fen zi mian yi xue za zhi bian ji bu |
| مواضيع طبية MeSH: | Adenoviridae/*genetics , DNA, Recombinant/*genetics , Genes, Viral/*genetics , Genetic Engineering/*methods , Orthohantavirus/*genetics, Cell Line ; Plasmids/genetics ; Promoter Regions, Genetic/genetics ; Transfection |
| مستخلص: | Aim: To construct a adenovirus vector containing the 0.7 kb fragment of S gene of Hantavirus, CAG promoter, and WPRE (mRNA-stabilizing post-transcriptional regulatory element from the woodchuck hepatitis virus). Methods: The fragments of CAG and WPRE were synthesized according to GenBank, and inserted into the plasmid pShuttle-S0.7 to create a transfer vector pShuttle-S0.7-CAG-WPRE. The S0.7-CAG-WPRE fragment was then cloned into Adeno-X; Viral DNA by PI-Sce I and I-Ceu I digestion. The recombinant adenovirus DNA was linearized by Pac I, transfected into HEK 293 cells via Lipofectamine; 2000, and the titer of the recombinant adenovirus was determined by Adeno-X; Rapid Titer Kit. The expressed product of S0.7-CAG-WPRE fragment was detected by immunofluorescence assay. Results: The sequence of S0.7-CAG-WPRE fragment was confirmed by sequencing, and the recombinant adenovirus containing S0.7-CAG-WPRE was titered at 10(13); pfu/L. HEK293 cells transfected with recombinant adenoviruses were detected positive by immunofluorescence assay using a specific mAb 1A8 against Hantavirus nucleoprotein. Conclusion: The recombinant adenovirus containing the 0.7 kb fragment of S segment of Hantavirus, CAG promoter, WPRE was constructed. |
| المشرفين على المادة: | 0 (DNA, Recombinant) |
| تواريخ الأحداث: | Date Created: 20100429 Date Completed: 20100809 Latest Revision: 20221207 |
| رمز التحديث: | 20240829 |
| PMID: | 20423645 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1007-8738 |
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