دورية أكاديمية
أعرض في EDS

A monobromobimane-based assay to measure the pharmacokinetic profile of reactive sulphide species in blood.

التفاصيل البيبلوغرافية
العنوان: A monobromobimane-based assay to measure the pharmacokinetic profile of reactive sulphide species in blood.
المؤلفون: Wintner EA; Ikaria, Seattle, WA, USA., Deckwerth TL, Langston W, Bengtsson A, Leviten D, Hill P, Insko MA, Dumpit R, VandenEkart E, Toombs CF, Szabo C
المصدر: British journal of pharmacology [Br J Pharmacol] 2010 Jun; Vol. 160 (4), pp. 941-57.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: England NLM ID: 7502536 Publication Model: Print Cited Medium: Internet ISSN: 1476-5381 (Electronic) Linking ISSN: 00071188 NLM ISO Abbreviation: Br J Pharmacol Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Wiley
Original Publication: London, Macmillian Journals Ltd.
مواضيع طبية MeSH: Analytic Sample Preparation Methods/*methods , Bridged Bicyclo Compounds/*chemistry , Sulfhydryl Compounds/*blood , Sulfhydryl Reagents/*chemistry , Sulfides/*blood , Sulfides/*pharmacokinetics, Animals ; Chromatography, High Pressure Liquid ; Fluorescent Dyes/chemistry ; Free Radicals/analysis ; Free Radicals/blood ; Free Radicals/chemistry ; Humans ; Hydrogen Sulfide/administration & dosage ; Hydrogen Sulfide/blood ; Hydrogen Sulfide/chemistry ; Hydrogen Sulfide/pharmacokinetics ; Kinetics ; Limit of Detection ; Male ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Fluorescence ; Spectrometry, Mass, Electrospray Ionization ; Sulfhydryl Compounds/analysis ; Sulfhydryl Compounds/chemistry ; Sulfides/administration & dosage ; Sulfides/chemistry ; Sulfides/therapeutic use ; Tandem Mass Spectrometry
مستخلص: Background and Purpose: Hydrogen sulphide (H(2)S) is a labile, endogenous metabolite of cysteine, with multiple biological roles. The development of sulphide-based therapies for human diseases will benefit from a reliable method of quantifying H(2)S in blood and tissues.
Experimental Approach: Concentrations of reactive sulphide in saline and freshly drawn whole blood were quantified by reaction with the thio-specific derivatization agent monobromobimane, followed by reversed-phase fluorescence HPLC and/or mass spectrometry. In pharmacokinetic studies, male rats were exposed either to intravenous infusions of sodium sulphide or to H(2)S gas inhalation, and levels of available blood sulphide were measured. Levels of dissolved H(2)S/HS(-) were concomitantly measured using an amperometric sensor.
Key Results: Monobromobimane was found to rapidly and quantitatively derivatize sulphide in saline or whole blood to yield the stable small molecule sulphide dibimane. Extraction and quantification of this bis-bimane derivative were validated via reversed-phase HPLC separation coupled to fluorescence detection, and also by mass spectrometry. Baseline levels of sulphide in blood were in the range of 0.4-0.9 microM. Intravenous administration of sodium sulphide solution (2-20 mg x kg(-1) x h(-1)) or inhalation of H(2)S gas (50-400 ppm) elevated reactive sulphide in blood in a dose-dependent manner. Each 1 mg x kg(-1) x h(-1) of sodium sulphide infusion into rats was found to be pharmacokinetically equivalent to approximately 30 ppm of H(2)S gas inhalation.
Conclusions and Implications: The monobromobimane derivatization method is a sensitive and reliable means to measure reactive sulphide species in whole blood. Using this method, we have established a bioequivalence between infused sodium sulphide and inhaled H(2)S gas.
References: Acta Pharmacol Sin. 2008 Jun;29(6):670-9. (PMID: 18501113)
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Dec 5;781(1-2):227-49. (PMID: 12450661)
Antioxid Redox Signal. 2003 Aug;5(4):493-501. (PMID: 13678538)
Trends Pharmacol Sci. 2007 Oct;28(10):501-5. (PMID: 17884186)
Anal Biochem. 2005 Jun 1;341(1):40-51. (PMID: 15866526)
FASEB J. 2007 Dec;21(14):4070-6. (PMID: 17634391)
Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):17977-82. (PMID: 17951430)
Chem Pharm Bull (Tokyo). 1992 Nov;40(11):3000-4. (PMID: 1477915)
Shock. 2006 Aug;26(2):154-61. (PMID: 16878023)
Life Sci. 2008 Oct 24;83(17-18):589-94. (PMID: 18790700)
Shock. 2008 Oct;30(4):359-64. (PMID: 18323742)
Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15560-5. (PMID: 17878306)
Nat Rev Drug Discov. 2007 Nov;6(11):917-35. (PMID: 17948022)
Sci Signal. 2009 Nov 10;2(96):ra72. (PMID: 19903941)
J Appl Physiol (1985). 2007 Jan;102(1):261-8. (PMID: 17038495)
Clin Sci (Lond). 2008 Aug;115(3):91-7. (PMID: 18315525)
Annu Rev Pharmacol Toxicol. 1992;32:109-34. (PMID: 1605565)
J Exp Biol. 2004 Oct;207(Pt 21):3667-79. (PMID: 15371475)
Gastroenterology. 2006 Jul;131(1):259-71. (PMID: 16831608)
Am J Physiol Heart Circ Physiol. 2007 Apr;292(4):H1953-60. (PMID: 17237242)
Eur J Cardiothorac Surg. 2008 May;33(5):906-13. (PMID: 18314343)
Nat Chem Biol. 2006 Apr;2(4):185-94. (PMID: 16547481)
Am J Physiol Regul Integr Comp Physiol. 2008 Jun;294(6):R1930-7. (PMID: 18417642)
Biochem J. 1982 Aug 15;206(2):267-77. (PMID: 7150244)
Br J Pharmacol. 2009 Nov;158(6):1442-53. (PMID: 19845680)
Am J Physiol Regul Integr Comp Physiol. 2008 Nov;295(5):R1479-85. (PMID: 18799635)
Anal Biochem. 1993 Nov 15;215(1):73-81. (PMID: 8297017)
Am J Physiol Heart Circ Physiol. 2008 Aug;295(2):H801-6. (PMID: 18567706)
J Anal Toxicol. 1991 May-Jun;15(3):148-50. (PMID: 1943059)
Shock. 2008 Jan;29(1):84-8. (PMID: 17621252)
Anal Biochem. 1981 Mar 1;111(2):357-65. (PMID: 7247030)
Trends Pharmacol Sci. 2008 Feb;29(2):84-90. (PMID: 18180046)
J Forensic Sci. 1988 Jan;33(1):217-22. (PMID: 3351460)
Anal Biochem. 1981 Jul 1;114(2):383-7. (PMID: 7304929)
Br J Pharmacol. 2008 Jan;153(1):100-9. (PMID: 17965734)
Br J Pharmacol. 2009 Jul;157(6):944-51. (PMID: 19422378)
J Forensic Sci. 1990 May;35(3):706-12. (PMID: 2348184)
Science. 2008 Oct 24;322(5901):587-90. (PMID: 18948540)
EMBO J. 2001 Nov 1;20(21):6008-16. (PMID: 11689441)
Br J Pharmacol. 2004 Dec;143(7):881-9. (PMID: 15504752)
المشرفين على المادة: 0 (Bridged Bicyclo Compounds)
0 (Fluorescent Dyes)
0 (Free Radicals)
0 (Sulfhydryl Compounds)
0 (Sulfhydryl Reagents)
0 (Sulfides)
V23UK0CYXL (monobromobimane)
YGR27ZW0Y7 (sodium sulfide)
YY9FVM7NSN (Hydrogen Sulfide)
تواريخ الأحداث: Date Created: 20100702 Date Completed: 20101103 Latest Revision: 20211020
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC2936000
DOI: 10.1111/j.1476-5381.2010.00704.x
PMID: 20590590
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-5381
DOI:10.1111/j.1476-5381.2010.00704.x