دورية أكاديمية
Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage.
| العنوان: | Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage. |
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| المؤلفون: | Liu SQ; Department of Pharmacy, National University of Singapore, Singapore., Tian Q, Hedrick JL, Po Hui JH, Ee PL, Yang YY |
| المصدر: | Biomaterials [Biomaterials] 2010 Oct; Vol. 31 (28), pp. 7298-307. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1995-> : Amsterdam : Elsevier Science Original Publication: [Guilford, England] : IPC Science and Technology Press, 1980- |
| مواضيع طبية MeSH: | Biocompatible Materials/*chemistry , Cartilage/*physiology , Cell Differentiation/*physiology , Chondrogenesis/*physiology , Hydrogels/*chemistry , Mesenchymal Stem Cells/*physiology , Peptides/*chemistry, Animals ; Biocompatible Materials/metabolism ; Cartilage/cytology ; Cattle ; Cell Survival ; Collagen/genetics ; Collagen/metabolism ; Drug Compounding ; Extracellular Matrix/chemistry ; Extracellular Matrix/metabolism ; Gene Expression Profiling ; Humans ; Hydrogels/chemical synthesis ; Hydrogels/metabolism ; Materials Testing ; Mesenchymal Stem Cells/cytology ; Peptides/chemical synthesis ; Peptides/genetics ; Peptides/metabolism ; Polyethylene Glycols/chemistry ; Polyethylene Glycols/metabolism |
| مستخلص: | In this study, a collagen mimetic peptide (CMP) containing a GFOGER sequence flanked by GPO repeat units (sequence: (GPO)(4)GFOGER(GPO)(4)GCG, CMP) was synthesized and chemically incorporated into a poly(ethylene glycol) (PEG) hydrogel through Michael addition chemistry. The PEG/collagen mimetic peptide hybrid hydrogel was used as a scaffold for encapsulation, proliferation and differentiation of human mesenchymal stem cells (hMSCs) into neocartilage/chondrocytes. Biophysical studies indicated that this peptide adopts stable triple helical conformation under simulated physiological conditions. Tetra hydroxyl PEG was functionalized to generate an acrylate group and reacted with the peptide, and hydrogels were formed in situ with the addition of cells and tetra sulfhydryl PEG via Michael addition. The effect of CMP on proliferation and chondrogenesis of hMSCs was investigated. The results demonstrated that PEG-CMP hydrogels provided a natural environment, which promoted chondrogenesis of hMSCs and enhanced secretion of cartilage specific ECM as compared to PEG hydrogels without the peptide. This was attributed to enhanced cell/matrix interactions via integrin beta1/GFOGER interactions. Further, chondrogenesis was found to be affected by matrix elasticity. Soft matrix induced a greater degree of chondrogenic differentiation; however, stiff matrix had an opposite effect, inhibiting chondrogenic differentiation probably due to limited mass transport. This soft PEG/CMP hydrogel shows promise as a biomimetic scaffold that provides a desirable environment for the chondrogenic differentiation of hMSCs and is useful for the repair of cartilage defects. |
| المشرفين على المادة: | 0 (Biocompatible Materials) 0 (Hydrogels) 0 (Peptides) 3WJQ0SDW1A (Polyethylene Glycols) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20100710 Date Completed: 20101110 Latest Revision: 20220331 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.biomaterials.2010.06.001 |
| PMID: | 20615545 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1878-5905 |
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| DOI: | 10.1016/j.biomaterials.2010.06.001 |