دورية أكاديمية
أعرض في EDS

Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors.

التفاصيل البيبلوغرافية
العنوان: Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors.
المؤلفون: Donofrio G; Dipartimento di Salute Animale, Sezione di Malattie Infettive, Facoltà di Medicina Veterinaria, via del Taglio 10, 43100 Parma, Italy. gaetano.donofrio@unipr.it, Capocefalo A, Franceschi V, Morini G, Del Bue M, Conti V, Cavirani S, Grolli S
المصدر: BMC cell biology [BMC Cell Biol] 2010 Sep 23; Vol. 11, pp. 73. Date of Electronic Publication: 2010 Sep 23.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100966972 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2121 (Electronic) Linking ISSN: 14712121 NLM ISO Abbreviation: BMC Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2000-
مواضيع طبية MeSH: Joint Diseases/*therapy , Lentivirus Infections/*therapy , Stromal Cells/*transplantation , Transduction, Genetic/*methods , Transfection/*methods, Adipose Tissue/cytology ; Animals ; Antigens, Viral/genetics ; Antigens, Viral/immunology ; Antigens, Viral/metabolism ; Cell Differentiation/genetics ; Cloning, Molecular ; Feasibility Studies ; Genetic Therapy ; Guided Tissue Regeneration ; Horses ; Immunity/genetics ; Immunization ; Joint Diseases/pathology ; Lentivirus Infections/genetics ; Lentivirus Infections/immunology ; Lentiviruses, Equine ; Stem Cell Transplantation ; Stromal Cells/immunology ; Stromal Cells/metabolism ; Stromal Cells/pathology
مستخلص: Background: Adipose-Derived Stromal Cells have been shown to have multiple lineage differentiation properties and to be suitable for tissues regeneration in many degenerative processes. Their use has been proposed for the therapy of joint diseases and tendon injuries in the horse. In the present report the genetic manipulation of Equine Adipose-Derived Stromal Cells has been investigated.
Results: Equine Adipose-Derived Stromal Cells were successfully virally transduced as well as transiently and stably transfected with appropriate parameters, without detrimental effect on their differentiation properties. Moreover, green fluorescent protein alone, fused to neo gene, or co-expressed as bi-cistronic reporter constructs, driven by viral and house-keeping gene promoters, were tested. The better expressed cassette was employed to stably transfect Adipose-Derived Stromal Cells for cell therapy purposes. Stably transfected Equine Adipose-Derived Stromal Cells with a heterologous secreted viral antigen were able to immunize horses upon injection into the lateral wall of the neck.
Conclusion: This study provides the methods to successfully transgenize Adipose-Derived Stromal Cells both by lentiviral vector and by transfection using optimized constructs with suitable promoters and reporter genes. In conclusion these findings provide a working platform for the delivery of potentially therapeutic proteins to the site of cells injection via transgenized Equine Adipose-Derived Stromal Cells.
References: Expert Opin Biol Ther. 2007 Nov;7(11):1621-6. (PMID: 17961087)
Adv Virus Res. 2000;55:599-609. (PMID: 11050959)
Nat Med. 2001 Jan;7(1):33-40. (PMID: 11135613)
Mol Ther. 2008 Dec;16(12):2002-10. (PMID: 18797452)
J Virol Methods. 2002 Mar;101(1-2):49-61. (PMID: 11849683)
PLoS One. 2010 Mar 18;5(3):e9750. (PMID: 20305783)
Circ Res. 2007 May 11;100(9):1249-60. (PMID: 17495232)
Vet Res Commun. 2009 Dec;33(8):811-21. (PMID: 19472068)
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15720-5. (PMID: 19805220)
J Cell Physiol. 2007 Nov;213(2):341-7. (PMID: 17620285)
J Biol Chem. 1990 Jun 25;265(18):10410-8. (PMID: 2113054)
Reproduction. 2008 Sep;136(3):361-6. (PMID: 18577555)
Methods Mol Biol. 2009;506:59-70. (PMID: 19110619)
J Cell Physiol. 2006 Jan;206(1):229-37. (PMID: 16021633)
Can Vet J. 2008 Oct;49(10):1009-17. (PMID: 19119371)
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3558-63. (PMID: 20133714)
Vaccine. 2008 Nov 11;26(48):6031-42. (PMID: 18812200)
PLoS One. 2008 Jul 02;3(7):e2532. (PMID: 18596956)
J Virol. 2005 Jul;79(13):8330-8. (PMID: 15956578)
Virology. 1989 Aug;171(2):623-5. (PMID: 2548334)
J Cell Physiol. 2009 Feb;218(2):237-45. (PMID: 18792913)
Trends Biotechnol. 2006 Apr;24(4):150-4. (PMID: 16488036)
Clin Vaccine Immunol. 2006 Jun;13(6):698-701. (PMID: 16760329)
المشرفين على المادة: 0 (Antigens, Viral)
تواريخ الأحداث: Date Created: 20100925 Date Completed: 20110321 Latest Revision: 20211020
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC2949624
DOI: 10.1186/1471-2121-11-73
PMID: 20863390
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2121
DOI:10.1186/1471-2121-11-73