دورية أكاديمية
New evaluations of circulating granulocyte and macrophage stem cells in healthy adults using conditioned media and recombinant human growth factors.
| العنوان: | New evaluations of circulating granulocyte and macrophage stem cells in healthy adults using conditioned media and recombinant human growth factors. |
|---|---|
| المؤلفون: | Vannier JP; Groupe d'Etude de la Différenciation Hématopoiétique et Tumorale, Faculté de Médecine de Rouen, Saint-Etienne-du-Rouvray, France., Sumereau-Dassin E, Jean P, Demares MJ, Lenormand B, Breton P, Tron P, Piguet H |
| المصدر: | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 1990; Vol. 44 (5), pp. 281-6. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8213295 Publication Model: Print Cited Medium: Print ISSN: 0753-3322 (Print) Linking ISSN: 07533322 NLM ISO Abbreviation: Biomed Pharmacother Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Paris : Editions Scientifiques Elsevier Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982- |
| مواضيع طبية MeSH: | Blood Cell Count/*methods , Granulocytes/*cytology , Macrophages/*cytology , Stem Cells/*cytology, Adult ; Blood Cell Count/drug effects ; Colony-Stimulating Factors/pharmacology ; Culture Media ; Female ; Humans ; Interleukin-3/pharmacology ; Male ; Middle Aged ; Monocytes |
| مستخلص: | Peripheral human blood contains granulo-monocyte (CFU-GM) and eosinophil (CFU-Eo) progenitors. In vitro, the number of colony forming units is thought to range from 0.1-14 per 2 x 10(5) plated cells. We show that the number of CFU-GM, Eo depends on culture methods. By modifying the usual assay method (using human umbilical cord plasma and the association of 2 stimulating conditioned media: activated lymphocyte conditioned medium and bone marrow fibroblast conditioned medium), we found different circulating CFU-GM, Eo numbers. The mean number of circulating CFU-GM, Eo in 107 healthy adults was 22.4 per 2 x 10(5) plated cells (range: 1-84). There was a slight difference between males (mean number: 23.6) and females (mean number: 20.4). The mean number of CFU-GM, Eo harvested on Percoll gradient was 123/ml of peripheral blood (range: 7-513). These results are far over those commonly reported in literature. This suggests that these latter results were probably underestimated. The use of recombinant human interleukin 3 and recombinant human GM (granulocyte-monocyte) colony-stimulating factors shows that CFU-GM, Eo numbers are found to be comparatively increased compared to that obtained with our modified method (using rhIL-3 alone), or that the size of those colonies is notably increased (using rhIL-3 + rhGM-CSF). |
| المشرفين على المادة: | 0 (Colony-Stimulating Factors) 0 (Culture Media) 0 (Interleukin-3) |
| تواريخ الأحداث: | Date Created: 19900101 Date Completed: 19910613 Latest Revision: 20191022 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0753-3322(90)90154-2 |
| PMID: | 2091811 |
| قاعدة البيانات: | MEDLINE |
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