دورية أكاديمية
Correlation between compositional and mechanical properties of human mesenchymal stem cell-collagen microspheres during chondrogenic differentiation.
| العنوان: | Correlation between compositional and mechanical properties of human mesenchymal stem cell-collagen microspheres during chondrogenic differentiation. |
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| المؤلفون: | Li CH; Department of Mechanical Engineering, The University of Hong Kong, Hong Kong Special Administrative Region, China., Chik TK, Ngan AH, Chan SC, Shum DK, Chan BP |
| المصدر: | Tissue engineering. Part A [Tissue Eng Part A] 2011 Mar; Vol. 17 (5-6), pp. 777-88. Date of Electronic Publication: 2010 Dec 07. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101466659 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1937-335X (Electronic) Linking ISSN: 19373341 NLM ISO Abbreviation: Tissue Eng Part A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc. |
| مواضيع طبية MeSH: | Microspheres*, Cell Differentiation/*drug effects , Chondrogenesis/*drug effects , Collagen/*pharmacology , Mesenchymal Stem Cells/*cytology , Mesenchymal Stem Cells/*drug effects, Animals ; Biomechanical Phenomena/drug effects ; Collagen Type I/metabolism ; Collagen Type II/metabolism ; Cross-Linking Reagents/metabolism ; Glycosaminoglycans/metabolism ; Humans ; Immunohistochemistry ; Male ; Mesenchymal Stem Cells/metabolism ; Mesenchymal Stem Cells/ultrastructure ; Middle Aged ; Rats ; Statistics, Nonparametric ; Time Factors |
| مستخلص: | Mesenchymal stem cell (MSC)-based engineering is promising for cartilage repair. However, the compositional mechanical relationship of the engineered structures has not been extensively studied, given the importance of such relationship in native cartilage tissues. In this study, a novel human MSC-collagen microsphere system was used to study the compositional mechanical relationship during in vitro chondrogenic differentiation using histological and biochemical methods and a microplate compression assay. The mechanical property was found positively correlating with newly deposited cartilage-relevant matrices, glycosaminoglycan, and type II collagen, and with the collagen crosslinker density, in agreement with the presence of thick collagen bundles upon structural characterization. On the other hand, the mechanical property negatively correlates with type I collagen and total collagen, suggesting that the initial collagen matrix scaffold of the microsphere system was being remodeled by the differentiating human MSCs. This study also demonstrated the application of a simple, sensitive, and nondestructive tool for monitoring the progression of chondrogenic differentiation of MSCs in tissue-engineered constructs and therefore contributes to future development of novel cartilage repair strategies. |
| المشرفين على المادة: | 0 (Collagen Type I) 0 (Collagen Type II) 0 (Cross-Linking Reagents) 0 (Glycosaminoglycans) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20101023 Date Completed: 20110602 Latest Revision: 20181201 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1089/ten.TEA.2010.0078 |
| PMID: | 20964578 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1937-335X |
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| DOI: | 10.1089/ten.TEA.2010.0078 |