دورية أكاديمية
Infection, viral dissemination, and antibody responses of rhesus macaques exposed to the human gammaretrovirus XMRV.
| العنوان: | Infection, viral dissemination, and antibody responses of rhesus macaques exposed to the human gammaretrovirus XMRV. |
|---|---|
| المؤلفون: | Onlamoon N; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA., Das Gupta J, Sharma P, Rogers K, Suppiah S, Rhea J, Molinaro RJ, Gaughan C, Dong B, Klein EA, Qiu X, Devare S, Schochetman G, Hackett J Jr, Silverman RH, Villinger F |
| المصدر: | Journal of virology [J Virol] 2011 May; Vol. 85 (9), pp. 4547-57. Date of Electronic Publication: 2011 Feb 16. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington Dc : American Society For Microbiology Original Publication: Baltimore, American Society for Microbiology. |
| مواضيع طبية MeSH: | Disease Models, Animal*, Antibodies, Viral/*blood , Macaca mulatta/*virology , Primate Diseases/*virology , Retroviridae Infections/*virology , Xenotropic murine leukemia virus-related virus/*immunology , Xenotropic murine leukemia virus-related virus/*pathogenicity, Animals ; CD4-Positive T-Lymphocytes/virology ; Chronic Disease ; Epithelial Cells/virology ; Humans ; Lymphocytes/virology ; Macrophages/virology ; Male ; Primate Diseases/immunology ; Primate Diseases/pathology ; Proviruses/isolation & purification ; Retroviridae Infections/immunology ; Retroviridae Infections/pathology ; Viral Tropism ; Viremia ; Virus Activation ; Virus Latency |
| مستخلص: | Xenotropic murine leukemia-related virus (XMRV) was identified in association with human prostate cancer and chronic fatigue syndrome. To examine the infection potential, kinetics, and tissue distribution of XMRV in an animal model, we inoculated five macaques with XMRV intravenously. XMRV established a persistent, chronic disseminated infection, with low transient viremia and provirus in blood lymphocytes during acute infection. Although undetectable in blood after about a month, XMRV viremia was reactivated at 9 months, confirming the chronicity of the infection. Furthermore, XMRV Gag was detected in tissues throughout, with wide dissemination throughout the period of monitoring. Surprisingly, XMRV infection showed organ-specific cell tropism, infecting CD4 T cells in lymphoid organs including the gastrointestinal lamina propria, alveolar macrophages in lung, and epithelial/interstitial cells in other organs, including the reproductive tract. Of note, in spite of the intravenous inoculation, extensive XMRV replication was noted in prostate during acute but not chronic infection even though infected cells were still detectable by fluorescence in situ hybridization (FISH) in prostate at 5 and 9 months postinfection. Marked lymphocyte activation occurred immediately postinfection, but antigen-specific cellular responses were undetectable. Antibody responses were elicited and boosted upon reexposure, but titers decreased rapidly, suggesting low antigen stimulation over time. Our findings establish a nonhuman primate model to study XMRV replication/dissemination, transmission, pathogenesis, immune responses, and potential future therapies. |
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| معلومات مُعتمدة: | P51 RR000165 United States RR NCRR NIH HHS; R01 CA103943 United States CA NCI NIH HHS; CA103943 United States CA NCI NIH HHS; DRR000165 United States PHS HHS |
| المشرفين على المادة: | 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20110218 Date Completed: 20110614 Latest Revision: 20211020 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3126245 |
| DOI: | 10.1128/JVI.02411-10 |
| PMID: | 21325416 |
| قاعدة البيانات: | MEDLINE |
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