دورية أكاديمية
Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance.
| العنوان: | Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance. |
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| المؤلفون: | Fadeel MA; US Naval Medical Research Unit No 3 NAMRU-3, Cairo, Egypt. moustafa.abdelfadeel.eg@med.navy.mil, House BL, Wasfy MM, Klena JD, Habashy EE, Said MM, Maksoud MA, Rahman BA, Pimentel G |
| المصدر: | Journal of infection in developing countries [J Infect Dev Ctries] 2011 Mar 21; Vol. 5 (3), pp. 169-75. Date of Electronic Publication: 2011 Mar 21. |
| نوع المنشور: | Comparative Study; Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Open Learning on Enteric Pathogens Country of Publication: Italy NLM ID: 101305410 Publication Model: Electronic Cited Medium: Internet ISSN: 1972-2680 (Electronic) Linking ISSN: 19722680 NLM ISO Abbreviation: J Infect Dev Ctries Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Italy?] : Open Learning on Enteric Pathogens |
| مواضيع طبية MeSH: | Clinical Laboratory Techniques/*methods , Typhoid Fever/*diagnosis, Adult ; Antibodies, Bacterial/blood ; Antigens, Bacterial ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Immunoglobulin A/blood ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Salmonella typhi/immunology ; Sensitivity and Specificity |
| مستخلص: | Introduction: Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. Methodology: Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses a mixture of somatic and flagellar antigens to detect the total antibody response against Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) infection. The levels of Ig isotype response (IgG, IgM and IgA) were also evaluated, and results were compared to those of TUBEX-TF and Typhidot commercial kits. Results: Of 234 culture-confirmed typhoid patients, the total Ig ELISA diagnosed 93% compared to 71% using Widal test. This sensitivity level (93%) is higher than that observed for the individual Ig ELISAs (IgG 75%; IgM 79%; IgA 57%) and the commercial tests TUBEX-TF (75%), Typhidot IgM (63%) and Typhidot IgG (28%). An agreement of 78% was achieved between the total Ig ELISA and Widal test. The average specificity of the ELISA was 96%. Using ELISA, up to 200 samples can be tested per run with cost per test at US$0.20. Conclusions: The developed ELISA shows superior sensitivity and specificity, when compared to Widal, TUBEX-TF and Typhidot assays, is more cost effective and allows higher throughput. This method is highly recommended for active surveillance studies or outbreak investigations of typhoid fever. |
| المشرفين على المادة: | 0 (Antibodies, Bacterial) 0 (Antigens, Bacterial) 0 (Immunoglobulin A) 0 (Immunoglobulin G) 0 (Immunoglobulin M) |
| تواريخ الأحداث: | Date Created: 20110330 Date Completed: 20110718 Latest Revision: 20191210 |
| رمز التحديث: | 20231215 |
| DOI: | 10.3855/jidc.1339 |
| PMID: | 21444985 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1972-2680 |
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| DOI: | 10.3855/jidc.1339 |