دورية أكاديمية
HILIC-NMR: toward the identification of individual molecular components in dissolved organic matter.
| العنوان: | HILIC-NMR: toward the identification of individual molecular components in dissolved organic matter. |
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| المؤلفون: | Woods GC; Department of Chemistry, University of Toronto, Ontario, Canada., Simpson MJ, Koerner PJ, Napoli A, Simpson AJ |
| المصدر: | Environmental science & technology [Environ Sci Technol] 2011 May 01; Vol. 45 (9), pp. 3880-6. Date of Electronic Publication: 2011 Apr 06. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0213155 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5851 (Electronic) Linking ISSN: 0013936X NLM ISO Abbreviation: Environ Sci Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington DC : American Chemical Society Original Publication: Easton, Pa. : American Chemical Society, c1967- |
| مواضيع طبية MeSH: | Humic Substances/*analysis , Magnetic Resonance Spectroscopy/*methods, Chromatography/methods ; Fluorescence ; Hydrophobic and Hydrophilic Interactions |
| مستخلص: | This article presents research targeted toward the isolation and detection of unique molecular structures from what is believed to be the world's most complex organic mixture: dissolved organic matter (DOM). Hydrophilic interaction chromatography (HILIC) was used to separate Suwannee River DOM (SRDOM) into 80 fractions, simplified to the extent that detection with nuclear magnetic resonance spectroscopy (NMR) results in many sharp signals that are indicative of individual compounds, some of which are identifiable with multidimensional NMR. Parallel factor analysis (PARAFAC) of fluorescence excitation-emission matrices (EEMs) was additionally employed on HILIC-simplified fractions to further confirm the effectiveness of the HILIC separations as well as draw insight into how structural characteristics relate to DOM fluorescence signals. Findings suggest that material believed to be derived from both cyclic and linear terpenoids was dominant in the most hydrophobic fractions as were the majority of the fluorescence signals, whereas hydrophilic material was highly correlated with carbohydrate-type structures as well as high contributions from amino acid fluorescence. NMR spectra of DOM, typically featureless mounds, are substantially more detailed with HILIC-simplified fractions to the point where hundreds of signals are present and 2D NMR correlations permit significant structural identifications. |
| التعليقات: | Comment in: Environ Sci Technol. 2011 Jul 1;45(13):5908-9; author reply 5910. (PMID: 21619034) |
| المشرفين على المادة: | 0 (Humic Substances) |
| تواريخ الأحداث: | Date Created: 20110408 Date Completed: 20110823 Latest Revision: 20191210 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/es103425s |
| PMID: | 21469703 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5851 |
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| DOI: | 10.1021/es103425s |