دورية أكاديمية
A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection.
| العنوان: | A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection. |
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| المؤلفون: | Lopez GH; Australian Red Cross Blood Service, Brisbane, Qld, Australia., Dean MM, Yasui K, Schuller RM, Hirayama F, Fung YL |
| المصدر: | Vox sanguinis [Vox Sang] 2012 Feb; Vol. 102 (2), pp. 171-4. Date of Electronic Publication: 2011 Aug 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Science Country of Publication: England NLM ID: 0413606 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1423-0410 (Electronic) Linking ISSN: 00429007 NLM ISO Abbreviation: Vox Sang Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2001- : Oxford, UK : Blackwell Science Original Publication: Basel : Karger |
| مواضيع طبية MeSH: | Antibodies/*blood , Fluorescent Antibody Technique/*methods , Isoantibodies/*blood , Isoantigens/*biosynthesis , Neutrophils/*immunology, Animals ; Antibodies/immunology ; Antibody Specificity ; Cattle ; Cell Line ; Humans ; Isoantibodies/immunology ; Isoantigens/blood ; Isoantigens/immunology ; Transfection |
| مستخلص: | There is an international need for a large-scale human neutrophils antigen (HNA) antibody screening platform to minimize the risk of antibody-mediated transfusion-related acute lung injury. However, sourcing a substantial, reliable source of HNA, as well as the scarcity of well-characterized HNA antisera for validating new screening platforms, remain as major obstacles. This short communication presents an improved protocol for the effective use of HNA-expressing KY cells as a screening platform using eight well-characterized HNA antisera of a single defined specificity. (© 2011 The Author(s). Vox Sanguinis © 2011 International Society of Blood Transfusion.) |
| المشرفين على المادة: | 0 (Antibodies) 0 (Isoantibodies) 0 (Isoantigens) |
| تواريخ الأحداث: | Date Created: 20110903 Date Completed: 20120815 Latest Revision: 20120118 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/j.1423-0410.2011.01532.x |
| PMID: | 21883271 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1423-0410 |
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| DOI: | 10.1111/j.1423-0410.2011.01532.x |