دورية أكاديمية
Increased NHE3 abundance and transport activity in renal proximal tubule of rats with heart failure.
| العنوان: | Increased NHE3 abundance and transport activity in renal proximal tubule of rats with heart failure. |
|---|---|
| المؤلفون: | Inoue BH; Heart Institute, University of São Paulo Medical School, Brazil., dos Santos L, Pessoa TD, Antonio EL, Pacheco BP, Savignano FA, Carraro-Lacroix LR, Tucci PJ, Malnic G, Girardi AC |
| المصدر: | American journal of physiology. Regulatory, integrative and comparative physiology [Am J Physiol Regul Integr Comp Physiol] 2012 Jan 01; Vol. 302 (1), pp. R166-74. Date of Electronic Publication: 2011 Oct 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901230 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1490 (Electronic) Linking ISSN: 03636119 NLM ISO Abbreviation: Am J Physiol Regul Integr Comp Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, Md. : American Physiological Society |
| مواضيع طبية MeSH: | Heart Failure/*metabolism , Kidney Tubules, Proximal/*metabolism , Sodium-Hydrogen Exchangers/*metabolism, Animals ; Biological Transport ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Disease Models, Animal ; Heart Failure/physiopathology ; Kidney Tubules, Proximal/physiopathology ; Male ; Models, Animal ; Phosphorylation ; RNA, Messenger/metabolism ; Rats ; Rats, Wistar ; Sodium-Hydrogen Exchanger 3 |
| مستخلص: | Heart failure (HF) is associated with a reduced effective circulating volume that drives sodium and water retention and extracellular volume expansion. We therefore hypothesized that Na(+)/H(+) exchanger isoform 3 (NHE3), the major apical transcellular pathway for sodium reabsorption in the proximal tubule, is upregulated in an experimental model of HF. HF was induced in male rats by left ventricle radiofrequency ablation. Sham-operated rats (sham) were used as controls. At 6 wk after surgery, HF rats exhibited cardiac dysfunction with a dramatic increase in left ventricular end-diastolic pressure. By means of stationary in vivo microperfusion and pH-dependent sodium uptake, we demonstrated that NHE3 transport activity was significantly higher in the proximal tubule of HF compared with sham rats. Increased NHE3 activity was paralleled by increased renal cortical NHE3 expression at both protein and mRNA levels. In addition, the baseline PKA-dependent NHE3 phosphorylation at serine 552 was reduced in renal cortical membranes of rats with HF. Collectively, these results suggest that NHE3 is upregulated in the proximal tubule of HF rats by transcriptional, translational, and posttranslational mechanisms. Enhanced NHE3-mediated sodium reabsorption in the proximal tubule may contribute to extracellular volume expansion and edema, the hallmark feature of HF. Moreover, our study emphasizes the importance of undertaking a cardiorenal approach to contain progression of cardiac disease. |
| المشرفين على المادة: | 0 (RNA, Messenger) 0 (Slc9a3 protein, rat) 0 (Sodium-Hydrogen Exchanger 3) 0 (Sodium-Hydrogen Exchangers) EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20111028 Date Completed: 20120326 Latest Revision: 20200930 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1152/ajpregu.00127.2011 |
| PMID: | 22031782 |
| قاعدة البيانات: | MEDLINE |
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