دورية أكاديمية
أعرض في EDS

A novel Rho-like protein TbRHP is involved in spindle formation and mitosis in trypanosomes.

التفاصيل البيبلوغرافية
العنوان: A novel Rho-like protein TbRHP is involved in spindle formation and mitosis in trypanosomes.
المؤلفون: Abbasi K; Department of Pathology, University of Cambridge, Cambridge, United Kingdom., DuBois KN, Leung KF, Dacks JB, Field MC
المصدر: PloS one [PLoS One] 2011; Vol. 6 (11), pp. e26890. Date of Electronic Publication: 2011 Nov 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Mitosis/*physiology , Protozoan Proteins/*metabolism , Spindle Apparatus/*metabolism , Trypanosoma brucei brucei/*cytology , Trypanosoma brucei brucei/*metabolism , rho GTP-Binding Proteins/*metabolism, Mitosis/genetics ; Protein Binding ; Signal Transduction/genetics ; Signal Transduction/physiology ; Trypanosoma brucei brucei/genetics ; rho GTP-Binding Proteins/genetics
مستخلص: Background: In animals and fungi Rho subfamily small GTPases are involved in signal transduction, cytoskeletal function and cellular proliferation. These organisms typically possess multiple Rho paralogues and numerous downstream effectors, consistent with the highly complex contributions of Rho proteins to cellular physiology. By contrast, trypanosomatids have a much simpler Rho-signaling system, and the Trypanosoma brucei genome contains only a single divergent Rho-related gene, TbRHP (Tb927.10.6240). Further, only a single RhoGAP-like protein (Tb09.160.4180) is annotated, contrasting with the >70 Rho GAP proteins from Homo sapiens. We wished to establish the function(s) of TbRHP and if Tb09.160.4180 is a potential GAP for this protein.
Methods/findings: TbRHP represents an evolutionarily restricted member of the Rho GTPase clade and is likely trypanosomatid restricted. TbRHP is expressed in both mammalian and insect dwelling stages of T. brucei and presents with a diffuse cytoplasmic location and is excluded from the nucleus. RNAi ablation of TbRHP results in major cell cycle defects and accumulation of multi-nucleated cells, coinciding with a loss of detectable mitotic spindles. Using yeast two hybrid analysis we find that TbRHP interacts with both Tb11.01.3180 (TbRACK), a homolog of Rho-kinase, and the sole trypanosome RhoGAP protein Tb09.160.4180, which is related to human OCRL.
Conclusions: Despite minimization of the Rho pathway, TbRHP retains an important role in spindle formation, and hence mitosis, in trypanosomes. TbRHP is a partner for TbRACK and an OCRL-related trypanosome Rho-GAP.
التعليقات: Erratum in: PLoS One. 2011;6(11). doi:10.1371/annotation/b4b38a99-0b4c-483a-ad21-4dd721974b97. Leung, Ka Fai [added].
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 090007 United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (Protozoan Proteins)
EC 3.6.5.2 (rho GTP-Binding Proteins)
تواريخ الأحداث: Date Created: 20111119 Date Completed: 20120410 Latest Revision: 20240216
رمز التحديث: 20240216
مُعرف محوري في PubMed: PMC3214021
DOI: 10.1371/journal.pone.0026890
PMID: 22096505
قاعدة البيانات: MEDLINE
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