دورية أكاديمية
[Mechanism of cleft palate in mice induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin].
| العنوان: | [Mechanism of cleft palate in mice induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. |
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| المؤلفون: | Pu YL; Chongqing Children's Hospital, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing Medical University, Chongqing 400014, China., Liu LL, Gan LQ, He XM, Fu YX |
| المصدر: | Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery [Zhonghua Zheng Xing Wai Ke Za Zhi] 2011 Nov; Vol. 27 (6), pp. 448-53. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Country of Publication: China NLM ID: 100957850 Publication Model: Print Cited Medium: Print ISSN: 1009-4598 (Print) Linking ISSN: 10094598 NLM ISO Abbreviation: Zhonghua Zheng Xing Wai Ke Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing Shih: Zhongguo yi xue ke xue yuan, Zhongguo xie he yi ke da xue, Zheng xing wai ke yan jiu suo, 2000- |
| مواضيع طبية MeSH: | Cleft Palate/*chemically induced , Polychlorinated Dibenzodioxins/*toxicity , Teratogens/*toxicity, Animals ; DNA Methylation ; Female ; Mice ; Mice, Inbred C57BL ; Pregnancy ; Promoter Regions, Genetic ; Signal Transduction ; Smad Proteins/metabolism ; Transforming Growth Factor beta3/metabolism |
| مستخلص: | Objective: To explore the mechanism of cleft palate in mice induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin (TCDD). Methods: On gestation day 10 (GD 10), 12 pregnant mice were randomly divided into two groups as the treated group and the control group with 6 mice in each group. The mice in the treated group received intragastric administration with 64 microg TCDD/kg, while the mice in the control group received equivalent corn oil. The embryos were examined under stereomicroscope to detect the incidence of cleft palate on GD 18.5. Another 18 pregnant mice were randomly divided into two groups (treated group and control group) on GD 10 with 9 pregnant mice in each group. Then each group was divided into 3 subgroups: GD 13.5, GD 14.5 and GD 15.5, with 3 pregnant mice in each subgroup. The palatal shelves were dissected from the embryos for RNA and DNA extraction on GD 13.5, GD 14.5 and GD 15.5. At last the expression of Smad 2-4 and Smad 7 mRNA was investigated by RT-PCR, and the TGF-beta3 promoter methylamine levels were investigated by methylation specific PCR (MSP). Results: The cleft palate mice model was established successfully by exposing pregnant C57BL/6J mice to TCDD. Total frequency of clefts was 100% in TCDD group, and the frequency of clefts was 0 in the control group. The relative expression of Smad 2 mRNA was 0.263 +/- 0.088, 0.296 +/- 0.016 and 0.159 +/- 0.027 in TCDD group, 0.180 +/- 0.042, 0.282 +/- 0.029 and 0.165 +/- 0.018 in control group. The relative expression of Smad 3 mRNA was 0.453 +/- 0.153, 0.551 +/- 0.160 and 0.328 +/- 0.049 in TCDD group, 0.375 +/- 0.126, 0.510 +/- 0.145 and 0.259 +/- 0.035 in control group. The relative expression of Smad 4 mRNA was 0.675 +/- 0.174, 0.577 +/- 0.070 and 0.396 +/- 0.066 in TCDD group, 0.557 +/- 0.138, 0.587 +/- 0.080 and 0.441 +/- 0.054 in control group. The relative expression of Smad 7 mRNA was 0.283 +/- 0.050, 0.320 +/- 0.068 and 0.169 +/- 0.045 in TCDD group, 0.207 +/- 0.043, 0.288 +/- 0.051 and 0.155 +/- 0.040 in control group. There was no significant difference between the TCDD treated mice and the control (P > 0.05). The TGF-beta3 promoters were at the un-methylation state both in the TCDD treated and control group. Conclusion: It suggests that TCDD could induce a stable formation of cleft palate, but it is not through the TGF-beta/Smad signaling nor through the modification of TGF-beta3 promoter methylation. |
| المشرفين على المادة: | 0 (Polychlorinated Dibenzodioxins) 0 (Smad Proteins) 0 (Teratogens) 0 (Tgfb3 protein, mouse) 0 (Transforming Growth Factor beta3) |
| تواريخ الأحداث: | Date Created: 20120202 Date Completed: 20130215 Latest Revision: 20161125 |
| رمز التحديث: | 20231215 |
| PMID: | 22292409 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1009-4598 |
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