دورية أكاديمية
Multisite performance evaluation of an enzyme-linked immunosorbent assay for detection of Giardia, Cryptosporidium, and Entamoeba histolytica antigens in human stool.
| العنوان: | Multisite performance evaluation of an enzyme-linked immunosorbent assay for detection of Giardia, Cryptosporidium, and Entamoeba histolytica antigens in human stool. |
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| المؤلفون: | Christy NC; Division of Infectious Diseases and International Health, University of Virginia School of Medicine, Charlottesville, Virginia, USA., Hencke JD, Escueta-De Cadiz A, Nazib F, von Thien H, Yagita K, Ligaba S, Haque R, Nozaki T, Tannich E, Herbein JF, Petri WA Jr |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 2012 May; Vol. 50 (5), pp. 1762-3. Date of Electronic Publication: 2012 Feb 29. |
| نوع المنشور: | Comparative Study; Evaluation Study; Journal Article; Multicenter Study; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Antigens, Protozoan/*analysis , Clinical Laboratory Techniques/*methods , Cryptosporidium/*isolation & purification , Entamoeba histolytica/*isolation & purification , Feces/*parasitology , Giardia/*isolation & purification , Parasitology/*methods, Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; Humans ; Infant ; Male ; Middle Aged ; Sensitivity and Specificity ; Young Adult |
| مستخلص: | A novel fecal antigen detection assay for fresh and frozen human samples that detects but does not differentiate Giardia spp, Cryptosporidium spp, and Entamoeba histolytica, the Tri-Combo parasite screen, was compared to three established enzyme-linked immunosorbent assays (ELISAs) at three international sites. It exhibited 97.9% sensitivity and 97.0% specificity, with positive and negative predictive values of 93.4% and 99.1%, respectively. The Tri-Combo test proved a reliable means to limit the use of individual parasite ELISAs to positive samples. |
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| معلومات مُعتمدة: | AI43596 United States AI NIAID NIH HHS; R01 AI043596 United States AI NIAID NIH HHS; R42 AI77171 United States AI NIAID NIH HHS; T32 AI007046 United States AI NIAID NIH HHS; R42 AI077171 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Antigens, Protozoan) |
| تواريخ الأحداث: | Date Created: 20120302 Date Completed: 20120716 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3347128 |
| DOI: | 10.1128/JCM.06483-11 |
| PMID: | 22378909 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-660X |
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| DOI: | 10.1128/JCM.06483-11 |