دورية أكاديمية
[Effect of pulsed electromagnetic field with different frequencies on the proliferation, apoptosis and migration of human ovarian cancer cells].
| العنوان: | [Effect of pulsed electromagnetic field with different frequencies on the proliferation, apoptosis and migration of human ovarian cancer cells]. |
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| المؤلفون: | Wang Q; Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China., Wu W, Chen X, He C, Liu X |
| المصدر: | Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi [Sheng Wu Yi Xue Gong Cheng Xue Za Zhi] 2012 Apr; Vol. 29 (2), pp. 291-5. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Sichuan Sheng sheng wu yi xue gong cheng xue hui Country of Publication: China NLM ID: 9426398 Publication Model: Print Cited Medium: Print ISSN: 1001-5515 (Print) Linking ISSN: 10015515 NLM ISO Abbreviation: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Chengdu : Sichuan Sheng sheng wu yi xue gong cheng xue hui Original Publication: Chengdu : Sichuan Sheng sheng wu yi xue gong cheng xue hui : Hua xi yi ke da xue : Chengdu ke ji da xue, |
| مواضيع طبية MeSH: | Apoptosis/*radiation effects , Cell Movement/*radiation effects , Cell Proliferation/*radiation effects , Electromagnetic Fields/*adverse effects , Ovarian Neoplasms/*pathology, Cell Line, Tumor ; Cystadenocarcinoma, Serous/pathology ; Female ; Humans |
| مستخلص: | Pulsed electromagnetic field (PEMF), a non-invasive physical treatment modality, is now used clinically to promote bone formation for osteoporosis. The patients after ectomy of ovarian cancer are easily complicated with osteoporosis. However, the safety parameters of PEMF treatment for the osteoporosis patients after resection of ovarian cancer remain unknown. Therefore, this study was designed to examine the effect of different frequency of PEMF on the proliferation, apoptosis and migration of human ovarian cancer cells (SKOV3 cells). Cultured SKOV3 cells were exposed to PEMF stimulation daily with radiation of 8 Hz, 16 Hz, 32 Hz and 64 Hz, respectively. We used sinusoidal waves with strength of 1 mT, twice a day with an interval of 12 hours. An exposure to the waves lasted 30 minutes, for 3 days, with those no PEMF stimulation serving as the control. The proliferation of cells was detected using EdU assay, and the apoptosis of cell was assessed with Annexin V-FITC fluorescence. The migration of cells was measured with the scratch wound assay. The data showed that exposure to PEMF of 1 mT, 8 Hz for 3 days could significantly inhibit the proliferation of SKOV3 cells and induce the apoptosis of the cells. The migrated distance and number were increased by 1 mT, 8 Hz or 32 Hz PEMF stimulation, but decreased by 1 mT, 16 Hz treatment. The results suggested that we should be careful about the safety of PEMF treatment and strictly choose the optical parameters in preventing or treating the osteoporosis of the patients after resection of ovarian cancer. |
| تواريخ الأحداث: | Date Created: 20120524 Date Completed: 20130926 Latest Revision: 20120523 |
| رمز التحديث: | 20231215 |
| PMID: | 22616177 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5515 |
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