دورية أكاديمية
A novel approach to the development of anticarcinogenic vaccines.
| العنوان: | A novel approach to the development of anticarcinogenic vaccines. |
|---|---|
| المؤلفون: | Glushkov AN; Institute of Human Ecology, Siberian Branch, Russian Academy of Sciences., Apalko SV, Filipenko ML, Matveeva VA, Bakulina AY, Lunin VG, Kostyanko MV |
| المصدر: | Acta naturae [Acta Naturae] 2010 Oct; Vol. 2 (4), pp. 105-11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Research University Higher School of Economics Country of Publication: Russia (Federation) NLM ID: 101525823 Publication Model: Print Cited Medium: Internet ISSN: 2075-8251 (Print) Linking ISSN: 20758251 NLM ISO Abbreviation: Acta Naturae Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : [Moscow] : National Research University Higher School of Economics Original Publication: Moscow : Park Media Ltd |
| مستخلص: | Human exposure to chemical carcinogens is an important etiological factor in cancer diseases. In this article, we will discuss a new approach to the development of anticarcinogenic vaccines. The main task in our research was to select a benzo[a]pyrene immunomimetic peptide considered as a hapten-specific component. For this purpose, we synthesized carcinogen-protein conjugates and prepared mono- and polyclonal antibodies to benzo[a]pyrene. Phage display technology was used to select the benzo[a]pyrene immunomimetic peptide, followed by an evaluation of the immunological properties of the obtained peptide. The obtained benzo[a]pyrene immunomimetic peptide could only simulate chemical carcinogens in the frame of the pIII protein. As a result, we prepared a recombinant protein composed of the benzo[a]pyrene immunomimetic peptide and pIII-encoding sequences. Using ELISA, we demonstrated that the recombinant protein specifically interacts with the anti-benzo[a]pyrene monoclonal antibody (mAB B2). Using molecular modeling, we predicted the 3-D structure of the mAB B2 active center and analyzed the characteristics of its interaction with different polycyclic aromatic hydrocarbons, as well as with the benzo[a]pyrene immunomimetic peptide. Thus, a comprehensive analysis of the results of the obtainment of hapten-specific components of anticarcinogenic vaccines allowed us to outline a strategy for future development in this direction. |
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| تواريخ الأحداث: | Date Created: 20120601 Date Completed: 20120823 Latest Revision: 20240322 |
| رمز التحديث: | 20240322 |
| مُعرف محوري في PubMed: | PMC3347585 |
| PMID: | 22649670 |
| قاعدة البيانات: | MEDLINE |
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