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Empowering biotechnology in southern Africa: establishment of a robust transformation platform for the production of transgenic industry-preferred cassava.

التفاصيل البيبلوغرافية
العنوان: Empowering biotechnology in southern Africa: establishment of a robust transformation platform for the production of transgenic industry-preferred cassava.
المؤلفون: Chetty CC; School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, South Africa., Rossin CB, Gruissem W, Vanderschuren H, Rey ME
المصدر: New biotechnology [N Biotechnol] 2013 Jan 25; Vol. 30 (2), pp. 136-43. Date of Electronic Publication: 2012 Jun 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101465345 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4347 (Electronic) Linking ISSN: 18716784 NLM ISO Abbreviation: N Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Industry* , Transformation, Genetic*, Biotechnology/*methods , Manihot/*genetics, Africa, Southern ; Germination ; Manihot/embryology ; Plant Shoots/growth & development ; Plants, Genetically Modified ; Regeneration ; Seeds/genetics
مستخلص: Knowledge and technology transfer to African laboratories and farmers is an important objective for achieving food security and sustainable crop production on the sub-Saharan African continent. Cassava (Manihot esculenta Crantz) is a vital source of calories for more than a billion people in developing countries, and its potential industrial use for starch and bioethanol in the tropics is increasingly being recognized. However, cassava production remains constrained by the susceptibility of the crop to several biotic and abiotic stresses. For more than a decade, biotechnology has been considered an attractive tool to improve cassava as it substantially circumvents the limitations of traditional breeding, which is particularly time-consuming and tedious because of the high heterozygosity of the crop. A major constraint to the development of biotechnological approaches for cassava improvement has been the lack of an efficient and robust transformation and regeneration system. Despite some success achieved in genetic modification of the model cassava cultivar Tropical Manihot Series (TMS), TMS 60444, in some European and U.S. laboratories, the lack of a reproducible and robust protocol has not allowed the establishment of a routine transformation system in sub-Saharan Africa. In this study, we optimized a robust and efficient protocol developed at ETH Zurich to successfully establish transformation of a commercially cultivated South African landrace, T200, and compared this with the benchmark model cultivar TMS 60444. Results from our study demonstrated high transformation rates for both T200 (23 transgenic lines from 100 friable embryogenic callus (FEC) clusters) compared with TMS 60444 (32 transgenic lines from 100 FEC clusters). The success in transforming landraces or farmer-preferred cultivars has been limited, and the high transformation rate of an industry-preferred landrace in this study is encouraging for a feasible transformation program for cassava improvement in South Africa (SA), which can potentially be extended to other countries in southern Africa. The successful establishment of a robust cassava transformation and regeneration system in SA demonstrates the relevance of technology transfer to sub-Saharan Africa and highlights the importance of developing suitable and reliable techniques before their transfer to laboratories offering less optimal conditions.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
تواريخ الأحداث: Date Created: 20120612 Date Completed: 20130702 Latest Revision: 20220321
رمز التحديث: 20240829
DOI: 10.1016/j.nbt.2012.04.006
PMID: 22683498
قاعدة البيانات: MEDLINE
الوصف
تدمد:1876-4347
DOI:10.1016/j.nbt.2012.04.006