دورية أكاديمية
أعرض في EDS

A system to enrich for primitive streak-derivatives, definitive endoderm and mesoderm, from pluripotent cells in culture.

التفاصيل البيبلوغرافية
العنوان: A system to enrich for primitive streak-derivatives, definitive endoderm and mesoderm, from pluripotent cells in culture.
المؤلفون: Vassilieva S; School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia., Goh HN, Lau KX, Hughes JN, Familari M, Rathjen PD, Rathjen J
المصدر: PloS one [PLoS One] 2012; Vol. 7 (6), pp. e38645. Date of Electronic Publication: 2012 Jun 11.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Cell Culture Techniques/*methods , Cell Differentiation/*physiology , Embryoid Bodies/*ultrastructure , Endoderm/*ultrastructure , Mesoderm/*ultrastructure , Pluripotent Stem Cells/*ultrastructure , Primitive Streak/*ultrastructure, Animals ; DNA Primers/genetics ; Flow Cytometry ; Gene Expression Profiling ; Horseradish Peroxidase/pharmacokinetics ; Mice ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Pluripotent Stem Cells/metabolism ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction
مستخلص: Two lineages of endoderm develop during mammalian embryogenesis, the primitive endoderm in the pre-implantation blastocyst and the definitive endoderm at gastrulation. This complexity of endoderm cell populations is mirrored during pluripotent cell differentiation in vitro and has hindered the identification and purification of the definitive endoderm for use as a substrate for further differentiation. The aggregation and differentiation of early primitive ectoderm-like (EPL) cells, resulting in the formation of EPL-cell derived embryoid bodies (EPLEBs), is a model of gastrulation that progresses through the sequential formation of primitive streak-like intermediates to nascent mesoderm and more differentiated mesoderm populations. EPL cell-derived EBs have been further analysed for the formation of definitive endoderm by detailed morphological studies, gene expression and a protein uptake assay. In comparison to embryoid bodies derived from ES cells, which form primitive and definitive endoderm, the endoderm compartment of embryoid bodies formed from EPL cells was comprised almost exclusively of definitive endoderm. Definitive endoderm was defined as a population of squamous cells that expressed Sox17, CXCR4 and Trh, which formed without the prior formation of primitive endoderm and was unable to endocytose horseradish peroxidase from the medium. Definitive endoderm formed in EPLEBs provides a substrate for further differentiation into specific endoderm lineages; these lineages can be used as research tools for understanding the mechanisms controlling lineage establishment and the nature of the transient intermediates formed. The similarity between mouse EPL cells and human ES cells suggests EPLEBs can be used as a model system for the development of technologies to enrich for the formation of human ES cell-derived definitive endoderm in the future.
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المشرفين على المادة: 0 (DNA Primers)
EC 1.11.1.- (Horseradish Peroxidase)
تواريخ الأحداث: Date Created: 20120616 Date Completed: 20121108 Latest Revision: 20211021
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC3372479
DOI: 10.1371/journal.pone.0038645
PMID: 22701686
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0038645