دورية أكاديمية
أعرض في EDS

cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit.

التفاصيل البيبلوغرافية
العنوان: cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit.
المؤلفون: Krapf D; Department of Veterinary and Animal Science, University of Massachusetts, Amherst, MA 01003, USA., Ruan YC, Wertheimer EV, Battistone MA, Pawlak JB, Sanjay A, Pilder SH, Cuasnicu P, Breton S, Visconti PE
المصدر: Developmental biology [Dev Biol] 2012 Sep 01; Vol. 369 (1), pp. 43-53. Date of Electronic Publication: 2012 Jun 30.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372762 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-564X (Electronic) Linking ISSN: 00121606 NLM ISO Abbreviation: Dev Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: San Diego, CA : Elsevier
Original Publication: New York.
مواضيع طبية MeSH: Epididymis/*growth & development , Epididymis/*metabolism , Spermatozoa/*metabolism, Animals ; Epididymis/cytology ; Gene Expression Regulation, Developmental ; Image Processing, Computer-Assisted ; Male ; Mice ; Mice, Knockout ; Organ Size ; Proto-Oncogene Proteins pp60(c-src)/genetics ; Proto-Oncogene Proteins pp60(c-src)/metabolism ; Sperm Capacitation/physiology ; Sperm Motility/physiology ; Spermatozoa/cytology
مستخلص: Changes that occur to mammalian sperm upon epididymal transit and maturation render these cells capable of moving progressively and capacitating. Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by phosphorylation and dephosphorylation cascades. Recent experiments have demonstrated that the Src family of kinases plays an important role in the regulation of these events. However, sperm from cSrc null mice display normal tyrosine phosphorylation associated with capacitation. We report here that, despite normal phosphorylation, sperm from cSrc null mice display a severe reduction in forward motility, and are unable to fertilize in vitro. Histological analysis of seminiferous tubules in the testes, caput and corpus epididymis do not reveal obvious defects. However, the cauda epididymis is significantly smaller, and expression of key transport proteins in the epithelial cells lining this region is reduced in cSrc null mice compared to wild type littermates. Although previously, we and others have shown the presence of cSrc in mature sperm from cauda epididymis, a closer evaluation indicates that this tyrosine kinase is not present in sperm from the caput epididymis, suggesting that this protein is acquired by sperm later during epididymal maturation. Consistent with this observation, cSrc is enriched in vesicles released by the epididymal epithelium known as epididymosomes. Altogether, these observations indicate that cSrc is essential for cauda epididymal development and suggest an essential role of this kinase in epididymal sperm maturation involving cSrc extracellular trafficking.
(Copyright © 2012 Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R01 HD044044 United States HD NICHD NIH HHS; DK38452 United States DK NIDDK NIH HHS; HD038082 United States HD NICHD NIH HHS; R01 HD045821 United States HD NICHD NIH HHS; HD45821 United States HD NICHD NIH HHS; R01 HD038082 United States HD NICHD NIH HHS; R01 HD040793 United States HD NICHD NIH HHS; P01 DK038452 United States DK NIDDK NIH HHS; R01HD44044 United States HD NICHD NIH HHS; HD40793 United States HD NICHD NIH HHS
المشرفين على المادة: EC 2.7.10.2 (Proto-Oncogene Proteins pp60(c-src))
تواريخ الأحداث: Date Created: 20120704 Date Completed: 20121016 Latest Revision: 20211021
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC3424100
DOI: 10.1016/j.ydbio.2012.06.017
PMID: 22750823
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-564X
DOI:10.1016/j.ydbio.2012.06.017