دورية أكاديمية
Application of quantitative real-time polymerase chain reaction for the diagnosis of toxoplasmosis and enzootic abortion of ewes.
| العنوان: | Application of quantitative real-time polymerase chain reaction for the diagnosis of toxoplasmosis and enzootic abortion of ewes. |
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| المؤلفون: | Gutierrez J; Instituto de Recursos Naturales y Agrobiología de Salamanca IRNASA-CSIC, Spain., O'Donovan J, Proctor A, Brady C, Marques PX, Worrall S, Nally JE, McElroy M, Bassett H, Fagan J, Maley S, Buxton D, Sammin D, Markey BK |
| المصدر: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2012 Sep; Vol. 24 (5), pp. 846-54. Date of Electronic Publication: 2012 Jul 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Country of Publication: United States NLM ID: 9011490 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1943-4936 (Electronic) Linking ISSN: 10406387 NLM ISO Abbreviation: J Vet Diagn Invest Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011-: Thousand Oaks, CA : Sage Original Publication: 1989-2004 : Columbia, MO : American Association of Veterinary Laboratory Diagnosticians |
| مواضيع طبية MeSH: | Abortion, Veterinary/*diagnosis , Chlamydophila/*isolation & purification , Chlamydophila Infections/*veterinary , Real-Time Polymerase Chain Reaction/*veterinary , Sheep Diseases/*parasitology , Toxoplasmosis, Animal/*diagnosis, Aborted Fetus/microbiology ; Abortion, Veterinary/microbiology ; Abortion, Veterinary/parasitology ; Animals ; Aqueous Humor/microbiology ; Chlamydophila/genetics ; Chlamydophila Infections/diagnosis ; Female ; Genotype ; Placenta/microbiology ; Pregnancy ; Real-Time Polymerase Chain Reaction/methods ; Reproducibility of Results ; Sheep ; Toxoplasma/genetics ; Toxoplasmosis, Animal/parasitology ; Vagina/microbiology |
| مستخلص: | Toxoplasma gondii and Chlamydophila abortus are the 2 most common infectious causes of ovine abortion worldwide. These obligate intracellular pathogens are associated with severe placentitis leading to abortion or stillbirth in pregnant ewes, and resulting in significant economic losses. The objectives of the current study were the development, validation, and application of a duplex real-time polymerase chain reaction (PCR) assay capable of quantifying the burden of infection by T. gondii and C. abortus in material submitted for diagnostic purposes. The validation was carried out using samples from ewes experimentally infected with these organisms. Based on the numbers of genome copies detected, an arbitrary cutoff level was established to correlate with significant pathological changes sufficient to give rise to abortion. When the PCR assay was applied to samples from 66 Irish farms with naturally occurring outbreaks of ovine abortion, toxoplasmosis and enzootic abortion of ewes (EAE) accounted for 14% and 20% of the farms, respectively, while on 6% of the farms, there was evidence of dual infection. When standard diagnostic techniques including histopathological examination, serological analysis, chlamydial antigen detection, and bacteriological culture, were used on samples from the same farms, toxoplasmosis was diagnosed in 17% of farms, and EAE in 12%; dual infection was diagnosed on 3% of the farms. In general, good agreement was found between the PCR and the standard methods. The duplex real-time PCR assay developed in this study has proved to be a very sensitive and rapid tool that might provide a valuable addition to the methods currently available for routine diagnosis of ovine abortions. |
| تواريخ الأحداث: | Date Created: 20120719 Date Completed: 20130114 Latest Revision: 20190522 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1177/1040638712452730 |
| PMID: | 22807509 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1943-4936 |
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| DOI: | 10.1177/1040638712452730 |