دورية أكاديمية
Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography for fatty acid profiling of cell wall phospholipids.
| العنوان: | Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography for fatty acid profiling of cell wall phospholipids. |
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| المؤلفون: | Bogusz S Jr; Institute of Chemistry, University of Campinas (Unicamp), Campinas, SP, Brazil., Hantao LW, Braga SC, de Matos França Vde C, da Costa MF, Hamer RD, Ventura DF, Augusto F |
| المصدر: | Journal of separation science [J Sep Sci] 2012 Sep; Vol. 35 (18), pp. 2438-44. Date of Electronic Publication: 2012 Aug 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101088554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-9314 (Electronic) Linking ISSN: 16159306 NLM ISO Abbreviation: J Sep Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim, Germany : Wiley-VCH, c2001- |
| مواضيع طبية MeSH: | Solid Phase Microextraction*, Cell Wall/*chemistry , Fatty Acids/*analysis , Glycerophospholipids/*chemistry , Mouth Mucosa/*cytology, Chromatography, Gas ; Humans |
| مستخلص: | The combination of solid-phase microextraction (SPME) with comprehensive two-dimensional gas chromatography is evaluated here for fatty acid (FA) profiling of the glycerophospholipid fraction from human buccal mucosal cells. A base-catalyzed derivatization reaction selective for polar lipids such as glycerophospholipid was adopted. SPME is compared to a miniaturized liquid-liquid extraction procedure for the isolation of FA methyl esters produced in the derivatization step. The limits of detection and limits of quantitation were calculated for each sample preparation method. Because of its lower values of limits of detection and quantitation, SPME was adopted. The extracted analytes were separated, detected, and quantified by comprehensive two-dimensional gas chromatography with flame ionization detection (FID). The combination of SPME and comprehensive two-dimensional gas chromatography with FID, using a selective derivatization reaction in the preliminary steps, proved to be a simple and fast procedure for FA profiling, and was successfully applied to the analysis of adult human buccal mucosal cells. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| المشرفين على المادة: | 0 (Fatty Acids) 0 (Glycerophospholipids) |
| تواريخ الأحداث: | Date Created: 20120822 Date Completed: 20130404 Latest Revision: 20120921 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/jssc.201200256 |
| PMID: | 22907894 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1615-9314 |
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| DOI: | 10.1002/jssc.201200256 |