دورية أكاديمية
Assignment of the backbone carbonyl resonances in (15)N-labelled proteins with (13)C at natural abundance by a 2D triple-resonance correlation technique.
| العنوان: | Assignment of the backbone carbonyl resonances in (15)N-labelled proteins with (13)C at natural abundance by a 2D triple-resonance correlation technique. |
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| المؤلفون: | Kristensen SM; Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen Ø, Denmark., Sørensen MD, Led JJ |
| المصدر: | Journal of biomolecular NMR [J Biomol NMR] 1995 Jun; Vol. 5 (4), pp. 411-4. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 9110829 Publication Model: Print Cited Medium: Print ISSN: 0925-2738 (Print) Linking ISSN: 09252738 NLM ISO Abbreviation: J Biomol NMR Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: <2005->: Leiden, The Netherlands : Springer Original Publication: Leiden, The Netherlands : ESCOM Science Publishers, c1991- |
| مستخلص: | A 2D NMR experiment for assignment of backbone carbon resonances in small and medium-sized (15)N-labelled proteins with (13)C at natural abundance is presented. The experiment is a two-dimensional variant of the HNCO triple-resonance experiment and is demonstrated by application to a 6 kDa protein at relatively low concentration (2 mM) and temperature (30°C). The experiment is particularly suitable for assignment of carbonyl resonances. |
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| تواريخ الأحداث: | Date Created: 20120823 Date Completed: 20121002 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/BF00182284 |
| PMID: | 22911559 |
| قاعدة البيانات: | MEDLINE |
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