دورية أكاديمية
Assignment of the backbone carbonyl resonances in (15)N-labelled proteins with (13)C at natural abundance by a 2D triple-resonance correlation technique.
العنوان: | Assignment of the backbone carbonyl resonances in (15)N-labelled proteins with (13)C at natural abundance by a 2D triple-resonance correlation technique. |
---|---|
المؤلفون: | Kristensen SM; Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen Ø, Denmark., Sørensen MD, Led JJ |
المصدر: | Journal of biomolecular NMR [J Biomol NMR] 1995 Jun; Vol. 5 (4), pp. 411-4. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 9110829 Publication Model: Print Cited Medium: Print ISSN: 0925-2738 (Print) Linking ISSN: 09252738 NLM ISO Abbreviation: J Biomol NMR Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Publication: <2005->: Leiden, The Netherlands : Springer Original Publication: Leiden, The Netherlands : ESCOM Science Publishers, c1991- |
مستخلص: | A 2D NMR experiment for assignment of backbone carbon resonances in small and medium-sized (15)N-labelled proteins with (13)C at natural abundance is presented. The experiment is a two-dimensional variant of the HNCO triple-resonance experiment and is demonstrated by application to a 6 kDa protein at relatively low concentration (2 mM) and temperature (30°C). The experiment is particularly suitable for assignment of carbonyl resonances. |
References: | Eur J Biochem. 1990 Dec 12;194(2):355-65. (PMID: 2176594) Biochemistry. 1986 Oct 7;25(20):5839-43. (PMID: 2431707) Biochemistry. 1989 Jan 10;28(1):230-6. (PMID: 2539856) J Am Chem Soc. 1988 Aug 1;110(18):6256-8. (PMID: 22148817) Biochemistry. 1990 May 15;29(19):4659-67. (PMID: 2372549) Methods Enzymol. 1994;239:363-92. (PMID: 7830591) Biochemistry. 1991 Oct 29;30(43):10457-66. (PMID: 1718420) Methods Enzymol. 1994;239:247-56. (PMID: 7830585) Biochemistry. 1992 Sep 15;31(36):8587-96. (PMID: 1356433) |
تواريخ الأحداث: | Date Created: 20120823 Date Completed: 20121002 Latest Revision: 20211021 |
رمز التحديث: | 20221213 |
DOI: | 10.1007/BF00182284 |
PMID: | 22911559 |
قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!